PMID- 23978190
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140819
LR  - 20131015
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 20
DP  - 2013 Oct 15
TI  - Automated digital microfluidic platform for magnetic-particle-based immunoassays 
      with optimization by design of experiments.
PG  - 9638-46
LID - 10.1021/ac401847x [doi]
AB  - We introduce an automated digital microfluidic (DMF) platform capable of 
      performing immunoassays from sample to analysis with minimal manual intervention. 
      This platform features (a) a 90 Pogo pin interface for digital microfluidic 
      control, (b) an integrated (and motorized) photomultiplier tube for 
      chemiluminescent detection, and (c) a magnetic lens assembly which focuses 
      magnetic fields into a narrow region on the surface of the DMF device, 
      facilitating up to eight simultaneous digital microfluidic magnetic separations. 
      The new platform was used to implement a three-level full factorial design of 
      experiments (DOE) optimization for thyroid-stimulating hormone immunoassays, 
      varying (1) the analyte concentration, (2) the sample incubation time, and (3) 
      the sample volume, resulting in an optimized protocol that reduced the detection 
      limit and sample incubation time by up to 5-fold and 2-fold, respectively, 
      relative to those from previous work. To our knowledge, this is the first report 
      of a DOE optimization for immunoassays in a microfluidic system of any format. We 
      propose that this new platform paves the way for a benchtop tool that is useful 
      for implementing immunoassays in near-patient settings, including community 
      hospitals, physicians' offices, and small clinical laboratories.
FAU - Choi, Kihwan
AU  - Choi K
AD  - Department of Chemistry, University of Toronto , 80 St. George Street, Toronto, 
      Ontario M5S 3H6, Canada.
FAU - Ng, Alphonsus H C
AU  - Ng AH
FAU - Fobel, Ryan
AU  - Fobel R
FAU - Chang-Yen, David A
AU  - Chang-Yen DA
FAU - Yarnell, Lyle E
AU  - Yarnell LE
FAU - Pearson, Elroy L
AU  - Pearson EL
FAU - Oleksak, Carl M
AU  - Oleksak CM
FAU - Fischer, Andrew T
AU  - Fischer AT
FAU - Luoma, Robert P
AU  - Luoma RP
FAU - Robinson, John M
AU  - Robinson JM
FAU - Audet, Julie
AU  - Audet J
FAU - Wheeler, Aaron R
AU  - Wheeler AR
LA  - eng
PT  - Journal Article
DEP - 20130826
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 2013/08/28 06:00
MHDA- 2013/08/28 06:01
CRDT- 2013/08/28 06:00
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2013/08/28 06:01 [medline]
AID - 10.1021/ac401847x [doi]
PST - ppublish
SO  - Anal Chem. 2013 Oct 15;85(20):9638-46. doi: 10.1021/ac401847x. Epub 2013 Aug 26.