PMID- 23978474
OWN - NLM
STAT- MEDLINE
DCOM- 20140607
LR  - 20171116
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 11
IP  - 3
DP  - 2013 Nov
TI  - Endothelial cells provide a niche for placental hematopoietic stem/progenitor 
      cell expansion through broad transcriptomic modification.
PG  - 1074-90
LID - S1873-5061(13)00110-4 [pii]
LID - 10.1016/j.scr.2013.07.010 [doi]
AB  - Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells 
      (HSCs). However, the number of HSCs in UCB is limited, and attempts to amplify 
      them in vitro remain inefficient. Several publications have documented 
      amplification of hematopoietic stem/progenitor cells (HSPCs) on endothelial or 
      mesenchymal cells, but the lack of homogeneity in culture conditions and HSC 
      definition impairs direct comparison of these results. We investigated the 
      ability of different feeder layers, mesenchymal progenitors (MPs) and endothelial 
      cells (ECs), to amplify hematopoietic stem/progenitor cells. Placental derived 
      HSPCs (defined as Lin(-)CD45(-/dim)CD34(+)CD38(-)CD90(+)) were maintained on 
      confluent feeder layers and the number of cells and their marker expression were 
      monitored over 21 days. Although both types of feeder layers supported 
      hematopoietic expansion, only endothelial cells triggered amplification of 
      Lin(-)CD45(-/dim)CD34(+)CD38(-)CD90(+) cells, which peaked at 14 days. The 
      amplified cells differentiated into all cell lineages, as attested by in vitro 
      colony-forming assays, and were capable of engraftment and multi-lineage 
      differentiation in sub-lethally irradiated mice. Mesenchymal progenitors promoted 
      amplification of CD38(+) cells, previously defined as precursors with more 
      limited differentiation potential. A competitive assay demonstrated that 
      hematopoietic stem/progenitor cells had a preference for interacting with 
      endothelial cells in vitro. Cytokine and transcriptomic analysis of both feeder 
      cell types identified differences in gene expression that correlated with 
      propensity of ECs and MPs to support hematopoietic cell amplification and 
      differentiation respectively. Finally, we used RNA sequencing of endothelial 
      cells and HSPCs to uncover relevant networks illustrating the complex interaction 
      between endothelial cells and HSPCs leading to stem/progenitor cell expansion.
CI  - (c) 2013.
FAU - Raynaud, Christophe M
AU  - Raynaud CM
AD  - Qatar Cardiovascular Research Center, Qatar Foundation, Qatar Science and 
      Technology Park, Doha, Qatar.
FAU - Butler, Jason M
AU  - Butler JM
FAU - Halabi, Najeeb M
AU  - Halabi NM
FAU - Ahmad, Faizzan S
AU  - Ahmad FS
FAU - Ahmed, Badereldeen
AU  - Ahmed B
FAU - Rafii, Shahin
AU  - Rafii S
FAU - Rafii, Arash
AU  - Rafii A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130809
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - 0 (Antigens, CD34)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - Fetal Blood/cytology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - Leukocyte Common Antigens/metabolism
MH  - Mice
MH  - Placenta/*cytology
MH  - Pregnancy
MH  - Signal Transduction
MH  - Stem Cell Niche
MH  - *Transcriptome
EDAT- 2013/08/28 06:00
MHDA- 2014/06/08 06:00
CRDT- 2013/08/28 06:00
PHST- 2013/01/09 00:00 [received]
PHST- 2013/07/17 00:00 [revised]
PHST- 2013/07/31 00:00 [accepted]
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2014/06/08 06:00 [medline]
AID - S1873-5061(13)00110-4 [pii]
AID - 10.1016/j.scr.2013.07.010 [doi]
PST - ppublish
SO  - Stem Cell Res. 2013 Nov;11(3):1074-90. doi: 10.1016/j.scr.2013.07.010. Epub 2013 
      Aug 9.