PMID- 23978538
OWN - NLM
STAT- MEDLINE
DCOM- 20140530
LR  - 20201209
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Linking)
VI  - 98
DP  - 2013 Sep
TI  - RTP801 immunoreactivity in retinal ganglion cells and its down-regulation in 
      cultured cells protect them from light and cobalt chloride.
PG  - 132-44
LID - S0361-9230(13)00136-6 [pii]
LID - 10.1016/j.brainresbull.2013.08.002 [doi]
AB  - RTP801, a stress-related protein, is activated by adverse environmental 
      conditions and inhibits the activity of mammalian target of rapamycin (mTOR) in 
      promoting oxidative stress-dependent cell death. RTP801 exists both in the 
      mammalian retina and the lens of the eye. Here, we observed RTP801 
      immunoreactivity in some retinal ganglion cells. Intravitreal injection of cobalt 
      chloride (CoCl2) to mimick hypoxia influenced retinal GFAP (glial fibrillary 
      acidic protein) and heme oxygenase-1 (HO-1) levels, but did not affect RTP801 
      immunoreactivity or mRNA content relative to GAPDH. However, RTP801 mRNA was 
      elevated when compared with Brn3a mRNA, suggesting that RTP801 is activated in 
      stressed Brn3a retinal ganglion cells. In cultures of RGC-5 cells, RTP801 
      immunoreactivity was located in the cytoplasm and partly present in the 
      mitochondria. An insult of blue light or CoCl2 increased RTP801 expression, which 
      was accompanied by cell death. However, in cultures where RTP801 mRNA was 
      down-regulated, the negative influence of blue light and CoCl2 was blunted. 
      Rapamycin nullified the CoCl2-induced up-regulation of RTP801 and attenuated cell 
      death. Moreover, rapamycin was non-toxic to RGC-5 cells, even at a high 
      concentration (10muM). The protective effect of rapamycin on RGC-5 cells caused by 
      the inhibition of RTP801 suggests that rapamycin might attenuate retinal ganglion 
      cell death in situ, as in glaucoma.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - del Olmo-Aguado, Susana
AU  - del Olmo-Aguado S
AD  - Fundacion de Investigacion Oftalmologica, Avda. Doctores Fernandez-Vega 34, 
      E-33012 Oviedo, Asturias, Spain.
FAU - Nunez-Alvarez, Claudia
AU  - Nunez-Alvarez C
FAU - Ji, Dan
AU  - Ji D
FAU - Manso, Alberto Garcia
AU  - Manso AG
FAU - Osborne, Neville N
AU  - Osborne NN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130824
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Ddit4 protein, rat)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Proton Ionophores)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 3G0H8C9362 (Cobalt)
RN  - 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone)
RN  - EVS87XF13W (cobaltous chloride)
SB  - IM
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Apoptosis/drug effects/radiation effects
MH  - Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
MH  - Cell Line, Transformed
MH  - Cobalt/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/*drug effects
MH  - Humans
MH  - Immunosuppressive Agents/pharmacology
MH  - Kidney/drug effects/metabolism
MH  - *Light
MH  - Mice
MH  - Proton Ionophores/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - Retina/cytology
MH  - *Retinal Ganglion Cells/drug effects/metabolism/radiation effects/ultrastructure
MH  - Transcription Factors
OTO - NOTNLM
OT  - Blue light
OT  - Chemical hypoxia
OT  - Ganglion cells
OT  - Neuroprotection
OT  - RTP801
OT  - Rapamycin
EDAT- 2013/08/28 06:00
MHDA- 2014/05/31 06:00
CRDT- 2013/08/28 06:00
PHST- 2013/06/25 00:00 [received]
PHST- 2013/08/13 00:00 [revised]
PHST- 2013/08/14 00:00 [accepted]
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2014/05/31 06:00 [medline]
AID - S0361-9230(13)00136-6 [pii]
AID - 10.1016/j.brainresbull.2013.08.002 [doi]
PST - ppublish
SO  - Brain Res Bull. 2013 Sep;98:132-44. doi: 10.1016/j.brainresbull.2013.08.002. Epub 
      2013 Aug 24.