PMID- 23979753
OWN - NLM
STAT- MEDLINE
DCOM- 20140514
LR  - 20211021
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 57
IP  - 11
DP  - 2013 Nov
TI  - Human herpesvirus 6 U69 kinase phosphorylates the methylenecyclopropane 
      nucleosides cyclopropavir, MBX 2168, and MBX 1616 to their monophosphates.
PG  - 5760-2
LID - 10.1128/AAC.00978-13 [doi]
AB  - Dihydroxymethyl and monohydroxymethyl methylenecyclopropane nucleosides are 
      effective inhibitors of both variants of human herpesvirus 6 (HHV-6). We 
      investigated involvement of HHV-6 U69 protein kinase in their mechanism of 
      action. Phosphorylation of the dihydroxymethyl analogue cyclopropavir and 
      monohydroxymethyl nucleosides with either a 6-ether moiety (MBX 2168) or a 
      6-thioether moiety (MBX 1616) with purified U69 was examined. All three compounds 
      were substrates of this viral kinase and had similar Michaelis-Menten kinetic 
      parameters.
FAU - Komazin-Meredith, Gloria
AU  - Komazin-Meredith G
AD  - Microbiotix, Inc., Worcester, Massachusetts, USA.
FAU - Cardinale, Steven C
AU  - Cardinale SC
FAU - Williams, John D
AU  - Williams JD
FAU - Peet, Norton P
AU  - Peet NP
FAU - Prichard, Mark N
AU  - Prichard MN
FAU - Bowlin, Terry L
AU  - Bowlin TL
LA  - eng
GR  - R43 AI082799/AI/NIAID NIH HHS/United States
GR  - R44 AI082799/AI/NIAID NIH HHS/United States
GR  - R44 AI 082799/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130826
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
RN  - 0 (Antiviral Agents)
RN  - 0 (Cyclopropanes)
RN  - 0 (Nucleosides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 5Z93L87A1R (Guanine)
RN  - EAO0TD9B13 (cyclopropavir)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Antiviral Agents/*chemistry
MH  - Baculoviridae/genetics
MH  - Cyclopropanes/*chemistry
MH  - Enzyme Assays
MH  - Guanine/*analogs & derivatives/chemistry
MH  - Herpesvirus 6, Human/chemistry/*enzymology
MH  - Humans
MH  - Kinetics
MH  - Nucleosides/*chemistry
MH  - Phosphorylation
MH  - Protein Kinases/*chemistry/genetics/isolation & purification
MH  - Recombinant Fusion Proteins/chemistry/genetics/isolation & purification
MH  - Substrate Specificity
MH  - Viral Proteins/*chemistry/genetics/isolation & purification
PMC - PMC3811273
EDAT- 2013/08/28 06:00
MHDA- 2014/05/16 06:00
PMCR- 2014/05/01
CRDT- 2013/08/28 06:00
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - AAC.00978-13 [pii]
AID - 00978-13 [pii]
AID - 10.1128/AAC.00978-13 [doi]
PST - ppublish
SO  - Antimicrob Agents Chemother. 2013 Nov;57(11):5760-2. doi: 10.1128/AAC.00978-13. 
      Epub 2013 Aug 26.