PMID- 23979788
OWN - NLM
STAT- MEDLINE
DCOM- 20140605
LR  - 20220317
IS  - 1439-4286 (Electronic)
IS  - 0018-5043 (Linking)
VI  - 45
IP  - 11
DP  - 2013 Oct
TI  - Serum preadipocyte factor-1 concentrations in females with obesity and type 2 
      diabetes mellitus: the influence of very low calorie diet, acute 
      hyperinsulinemia, and fenofibrate treatment.
PG  - 820-6
LID - 10.1055/s-0033-1353210 [doi]
AB  - Appropriate differentiation capacity of adipose tissue significantly affects its 
      ability to store lipids and to protect nonadipose tissues against lipid spillover 
      and development of insulin resistance. Preadipocyte factor-1 (Pref-1) is an 
      important negative regulator of preadipocyte differentiation. The aim of our 
      study was to explore the changes in circulating Pref-1 concentrations in female 
      subjects with obesity (OB) (n=19), females with obesity and type 2 diabetes 
      mellitus (T2DM) (n=22), and sex- and age-matched healthy control subjects (C) 
      (n=22), and to study its modulation by very low calorie diet (VLCD), acute 
      hyperinsulinemia during isoglycemic-hyperinsulinemic clamp, and 3 months' 
      treatment with PPAR-alpha agonist fenofibrate. At baseline, serum Pref-1 
      concentrations were significantly higher in patients with T2DM compared to 
      control group, while only nonsignificant trend towards higher levels was observed 
      in OB group. 3 weeks of VLCD decreased Pref-1 levels in both OB and T2DM group, 
      whereas 3 months of fenofibrate treatment had no significant effect. 
      Hyperinsulinemia during the clamp significantly suppressed Pref-1 levels in both 
      C and T2DM subjects and this suppression was unaffected by fenofibrate treatment. 
      In a combined population of all groups, circulating Pref-1 levels correlated 
      positively with insulin, leptin and glucose levels and HOMA (homeostasis model 
      assessment) index. We conclude that elevated Pref-1 concentrations in T2DM 
      subjects may contribute to impaired adipose tissue differentiation capacity 
      associated with insulin resistance in obese patients with T2DM. The decrease of 
      Pref-1 levels after VLCD may be involved in the improvement of metabolic status 
      and the amelioration of insulin resistance in T2DM patients.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Kavalkova, P
AU  - Kavalkova P
AD  - Third Department of Medicine, General University Hospital and First Faculty of 
      Medicine, Charles University, Prague, Czech Republic.
FAU - Touskova, V
AU  - Touskova V
FAU - Roubicek, T
AU  - Roubicek T
FAU - Trachta, P
AU  - Trachta P
FAU - Urbanova, M
AU  - Urbanova M
FAU - Drapalova, J
AU  - Drapalova J
FAU - Haluzikova, D
AU  - Haluzikova D
FAU - Mraz, M
AU  - Mraz M
FAU - Novak, D
AU  - Novak D
FAU - Matoulek, M
AU  - Matoulek M
FAU - Lacinova, Z
AU  - Lacinova Z
FAU - Haluzik, M
AU  - Haluzik M
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130826
PL  - Germany
TA  - Horm Metab Res
JT  - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et 
      metabolisme
JID - 0177722
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DLK1 protein, human)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (PPAR alpha)
RN  - U202363UOS (Fenofibrate)
SB  - IM
MH  - Anthropometry
MH  - Body Weight
MH  - Calcium-Binding Proteins
MH  - *Caloric Restriction
MH  - Diabetes Mellitus, Type 2/*blood/complications/*drug therapy
MH  - Female
MH  - Fenofibrate/*therapeutic use
MH  - Glucose Clamp Technique
MH  - Humans
MH  - Hyperinsulinism/*blood/drug therapy
MH  - Intercellular Signaling Peptides and Proteins/*blood
MH  - Membrane Proteins/*blood
MH  - Middle Aged
MH  - Obesity/*blood/complications/*diet therapy/drug therapy
MH  - PPAR alpha/agonists/metabolism
EDAT- 2013/08/28 06:00
MHDA- 2014/06/06 06:00
CRDT- 2013/08/28 06:00
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2014/06/06 06:00 [medline]
AID - 10.1055/s-0033-1353210 [doi]
PST - ppublish
SO  - Horm Metab Res. 2013 Oct;45(11):820-6. doi: 10.1055/s-0033-1353210. Epub 2013 Aug 
      26.