PMID- 23983038
OWN - NLM
STAT- MEDLINE
DCOM- 20141006
LR  - 20140212
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Linking)
VI  - 522
IP  - 5
DP  - 2014 Apr 1
TI  - Brain-derived neurotrophic factor: mRNA expression and protein distribution in 
      the brain of the teleost Nothobranchius furzeri.
PG  - 1004-30
LID - 10.1002/cne.23457 [doi]
AB  - BDNF (brain-derived neurotrophic factor) is a member of the neurotrophin family 
      and it is implicated in regulating brain development and function. The BDNF gene 
      organization and coding sequence are conserved in all vertebrates. The present 
      survey was conducted in a teleost fish, Nothobranchius furzeri, because it is an 
      emerging model of aging studies due to its short lifespan and shows the high rate 
      of adult neurogenesis typical of anamniotes. The present survey reports: 1) the 
      identification and characterization of the cDNA fragment encoding BDNF protein, 
      and 2) the localization of BDNF in the whole brain. BDNF mRNA expression was 
      assessed by in situ hybridization, by employing an antisense RNA probe; BDNF 
      protein was detected by employing a sensitive immunohistochemical technique, 
      along with highly specific affinity-purified antibodies to BDNF. Both BDNF mRNA 
      and protein were detected in neurons and glial cells of all regions of the brain 
      of N. furzeri. Interestingly, BDNF was localized also in brain areas involved in 
      adult neurogenic activities, suggesting a specific role for this neurotrophic 
      factor in controlling cell proliferation. These results provide baseline 
      information for future studies concerning BDNF involvement in the aging processes 
      of the teleost brain.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - D'Angelo, Livia
AU  - D'Angelo L
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      Federico II, Napoli, Italy; Biology of Ageing, Leibniz Institute for Age 
      Research, Fritz-Lipmann Institute, Jena, Germany.
FAU - De Girolamo, Paolo
AU  - De Girolamo P
FAU - Lucini, Carla
AU  - Lucini C
FAU - Terzibasi, Eva Tozzini
AU  - Terzibasi ET
FAU - Baumgart, Mario
AU  - Baumgart M
FAU - Castaldo, Luciana
AU  - Castaldo L
FAU - Cellerino, Alessandro
AU  - Cellerino A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Brain/anatomy & histology/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics/*metabolism
MH  - Fishes
MH  - Fructose-Bisphosphate Aldolase/genetics/metabolism
MH  - RNA, Messenger/*metabolism
OTO - NOTNLM
OT  - BDNF
OT  - Nothobranchius furzeri
OT  - brain
OT  - phylogenesis
EDAT- 2013/08/29 06:00
MHDA- 2014/10/07 06:00
CRDT- 2013/08/29 06:00
PHST- 2012/12/21 00:00 [received]
PHST- 2013/03/28 00:00 [revised]
PHST- 2013/08/06 00:00 [accepted]
PHST- 2013/08/29 06:00 [entrez]
PHST- 2013/08/29 06:00 [pubmed]
PHST- 2014/10/07 06:00 [medline]
AID - 10.1002/cne.23457 [doi]
PST - ppublish
SO  - J Comp Neurol. 2014 Apr 1;522(5):1004-30. doi: 10.1002/cne.23457.