PMID- 23983128
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20191210
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Linking)
VI  - 70
IP  - 6
DP  - 2014 Jun
TI  - Selection of a nucleopolyhedrovirus isolate from Helicoverpa armigera as the 
      basis for a biological insecticide.
PG  - 967-76
LID - 10.1002/ps.3637 [doi]
AB  - BACKGROUND: The cotton bollworm, Helicoverpa armigera, is an insect that causes 
      damage in a wide range of crops in Spain. Seven isolates of H. armigera single 
      nucleopolyhedrovirus (HearSNPV) from the Iberian Peninsula were subjected to 
      molecular and biological characterization and compared with a Chinese genotype 
      (HearSNPV-G4). RESULTS: The estimated sizes of the Iberian genomes varied between 
      116.2 and 132.4 kb, compared to 131.4 kb of the HearSNPV-G4 reference genome. 
      Phylogenetic analysis based on the lef-8, lef-9 and polh genes revealed that the 
      Iberian strains were more closely related to one another than to other HearSNPV 
      isolates. Occlusion body (OB) concentration-mortality responses (LC50 values) did 
      not differ significantly among Iberian isolates when tested against a Helicoverpa 
      armigera colony from Oxford (UK). Despite being the fastest killing isolate, 
      HearSNPV-SP1 was as productive as isolates with lower virulence, with an average 
      yield of 3.1 x 10(9) OBs larva(-1) . OBs of HearSNPV-SP1 and HearSNPV-G4 were 
      similarly pathogenic against a recently established colony from southern Spain, 
      although HearSNPV-SP1 was faster killing than HearSNPV-G4 against a range of 
      instars. CONCLUSION: The insecticidal properties of HearSNPV-SP1 mean that this 
      strain is likely to prove useful as the basis for a biological insecticide for 
      control of Helicoverpa armigera in Spain.
CI  - (c) 2013 Society of Chemical Industry.
FAU - Arrizubieta, Maite
AU  - Arrizubieta M
AD  - Bioinsecticidas Microbianos, Instituto de Agrobiotecnologia, CSIC-UPNA, Mutilva 
      Baja, Navarra, Spain.
FAU - Williams, Trevor
AU  - Williams T
FAU - Caballero, Primitivo
AU  - Caballero P
FAU - Simon, Oihane
AU  - Simon O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131013
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
RN  - 0 (Biological Control Agents)
SB  - IM
MH  - Animals
MH  - *Biological Control Agents
MH  - Genome
MH  - Insect Control/methods
MH  - Moths/*virology
MH  - Nucleopolyhedroviruses/*genetics/isolation & purification/pathogenicity
MH  - Phylogeny
MH  - Portugal
MH  - Spain
OTO - NOTNLM
OT  - Helicoverpa armigera
OT  - REN analysis
OT  - baculovirus
OT  - ingredient active production
OT  - insecticidal properties
OT  - physical map
EDAT- 2013/08/29 06:00
MHDA- 2015/01/06 06:00
CRDT- 2013/08/29 06:00
PHST- 2013/05/03 00:00 [received]
PHST- 2013/08/08 00:00 [revised]
PHST- 2013/08/23 00:00 [accepted]
PHST- 2013/08/29 06:00 [entrez]
PHST- 2013/08/29 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - 10.1002/ps.3637 [doi]
PST - ppublish
SO  - Pest Manag Sci. 2014 Jun;70(6):967-76. doi: 10.1002/ps.3637. Epub 2013 Oct 13.