PMID- 23985908
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 51
IP  - 11
DP  - 2013 Nov
TI  - Classical molecular tests using urine samples as a potential screening tool for 
      human papillomavirus detection in human immunodeficiency virus-infected women.
PG  - 3688-93
LID - 10.1128/JCM.01302-13 [doi]
AB  - Human papillomavirus (HPV) is the main risk factor associated with the 
      development of cervical cancer (CC); however, there are other factors, such as 
      immunosuppression caused by the human immunodeficiency virus (HIV), that favor 
      progression of the illness. This study was thus aimed at evaluating the 
      functionality of classical PCR-based molecular tests for the generic 
      identification of HPV DNA (GP5+/GP6+, MY09/MY11, and pU1M/2R primers, 
      individually or in combination) using cervical and urine samples from 194 
      HIV-positive women. Infected samples were tested with type-specific primers for 
      six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types 
      (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical 
      samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type 
      in the cervical and urine samples, with higher rates of multiple infections than 
      single infections detected in such samples. HPV DNA detection by PCR (mainly with 
      the pU1M/2R primer set) in urine samples was positively associated with abnormal 
      cytological findings (atypical squamous cells of undetermined 
      significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined 
      that the operative characteristics for detection of cytological abnormalities 
      were similar for cervical and urine samples. This suggested using PCR for the 
      detection of HPV DNA in urine samples as a potential screening strategy for CC 
      prevention in future prevention and control programs along with currently 
      implemented strategies for reducing the impact of the disease, i.e., urine 
      samples are economical, are easy to collect, have wide acceptability among women, 
      and have operative characteristics similar to those of cervical samples.
FAU - Munoz, Marina
AU  - Munoz M
AD  - Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia.
FAU - Camargo, Milena
AU  - Camargo M
FAU - Soto-De Leon, Sara C
AU  - Soto-De Leon SC
FAU - Sanchez, Ricardo
AU  - Sanchez R
FAU - Pineda-Pena, Andrea C
AU  - Pineda-Pena AC
FAU - Perez-Prados, Antonio
AU  - Perez-Prados A
FAU - Patarroyo, Manuel E
AU  - Patarroyo ME
FAU - Patarroyo, Manuel A
AU  - Patarroyo MA
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130828
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Cervix Uteri/virology
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics
MH  - Female
MH  - HIV Infections/*complications
MH  - Humans
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Papillomavirus Infections/*diagnosis
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Urine/*virology
MH  - Virology/*methods
PMC - PMC3889752
EDAT- 2013/08/30 06:00
MHDA- 2014/04/01 06:00
PMCR- 2014/05/01
CRDT- 2013/08/30 06:00
PHST- 2013/08/30 06:00 [entrez]
PHST- 2013/08/30 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - JCM.01302-13 [pii]
AID - 01302-13 [pii]
AID - 10.1128/JCM.01302-13 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2013 Nov;51(11):3688-93. doi: 10.1128/JCM.01302-13. Epub 2013 
      Aug 28.