PMID- 23986480
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20131031
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 126
IP  - Pt 21
DP  - 2013 Nov 1
TI  - Embryonic stem cells stably expressing BDNF-GFP exhibit a BDNF-release-dependent 
      enhancement of neuronal differentiation.
PG  - 5062-73
LID - 10.1242/jcs.135384 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is known to be a crucial regulator of 
      neuronal survival and synaptic plasticity in the mammalian brain. Furthermore, 
      BDNF positively influences differentiation of embryonic neural precursors, as 
      well as that of neural stem cells from adult neurogenic niches. To study the 
      impact of cell-released BDNF on neural differentiation of embryonic stem cells 
      (ESCs), which represent an attractive source for cell transplantation studies, we 
      have generated mouse ESC clones overexpressing BDNF-GFP by use of knock-in 
      technology. After neural differentiation in vitro, we observed that ESC clones 
      overexpressing BDNF-GFP gave rise to an increased number of neurons as compared 
      to control ESCs. Neurons derived from BDNF-GFP-expressing ESCs harbored a more 
      complex dendritic morphology and differentiated into the GABAergic lineage more 
      than controls. Moreover, we show that ESC-derived neurons released BDNF-GFP in an 
      activity-dependent manner and displayed similar electrophysiological properties 
      as cortical neurons. Thus, our study describes the generation of ESCs stably 
      overexpressing BDNF-GFP, which are ideally suited to investigate the ameliorating 
      effects of BDNF in cell transplantation studies of various neuropathological 
      conditions.
FAU - Leschik, Julia
AU  - Leschik J
AD  - Institute of Physiological Chemistry, University Medical Center of the Johannes 
      Gutenberg University, D-55128 Mainz, Germany.
FAU - Eckenstaler, Robert
AU  - Eckenstaler R
FAU - Nieweg, Katja
AU  - Nieweg K
FAU - Lichtenecker, Petra
AU  - Lichtenecker P
FAU - Brigadski, Tanja
AU  - Brigadski T
FAU - Gottmann, Kurt
AU  - Gottmann K
FAU - Lessmann, Volkmar
AU  - Lessmann V
FAU - Lutz, Beat
AU  - Lutz B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130828
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Embryonic Stem Cells/*cytology/metabolism
MH  - Green Fluorescent Proteins/genetics/*metabolism
MH  - Mice
MH  - Neurons/*cytology/metabolism
OTO - NOTNLM
OT  - Activity-dependent release
OT  - BDNF
OT  - Brain-derived neurotrophic factor
OT  - Embryonic stem cells
OT  - Neural differentiation
EDAT- 2013/08/30 06:00
MHDA- 2014/06/04 06:00
CRDT- 2013/08/30 06:00
PHST- 2013/08/30 06:00 [entrez]
PHST- 2013/08/30 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
AID - jcs.135384 [pii]
AID - 10.1242/jcs.135384 [doi]
PST - ppublish
SO  - J Cell Sci. 2013 Nov 1;126(Pt 21):5062-73. doi: 10.1242/jcs.135384. Epub 2013 Aug 
      28.