PMID- 23987132
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20240321
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 180
IP  - 4
DP  - 2013 Oct
TI  - Gap junction communication and the propagation of bystander effects induced by 
      microbeam irradiation in human fibroblast cultures: the impact of radiation 
      quality.
PG  - 367-75
LID - 10.1667/RR3111.1 [doi]
AB  - Understanding the mechanisms underlying the bystander effects of low doses/low 
      fluences of low- or high-linear energy transfer (LET) radiation is relevant to 
      radiotherapy and radiation protection. Here, we investigated the role of 
      gap-junction intercellular communication (GJIC) in the propagation of stressful 
      effects in confluent normal human fibroblast cultures wherein only 0.036-0.144% 
      of cells in the population were traversed by primary radiation tracks. Confluent 
      cells were exposed to graded doses from monochromatic 5.35 keV X ray (LET ~6 
      keV/mum), 18.3 MeV/u carbon ion (LET ~103 keV/mum), 13 MeV/u neon ion (LET ~380 
      keV/mum) or 11.5 MeV/u argon ion (LET ~1,260 keV/mum) microbeams in the presence or 
      absence of 18-alpha-glycyrrhetinic acid (AGA), an inhibitor of GJIC. After 4 h 
      incubation at 37 degrees C, the cells were subcultured and assayed for micronucleus (MN) 
      formation. Micronuclei were induced in a greater fraction of cells than expected 
      based on the fraction of cells targeted by primary radiation, and the effect 
      occurred in a dose-dependent manner with any of the radiation sources. 
      Interestingly, MN formation for the heavy-ion microbeam irradiation in the 
      absence of AGA was higher than in its presence at high mean absorbed doses. In 
      contrast, there were no significant differences in cell cultures exposed to X-ray 
      microbeam irradiation in presence or absence of AGA. This showed that the 
      inhibition of GJIC depressed the enhancement of MN formation in bystander cells 
      from cultures exposed to high-LET radiation but not low-LET radiation. Bystander 
      cells recipient of growth medium harvested from 5.35 keV X-irradiated cultures 
      experienced stress manifested in the form of excess micronucleus formation. 
      Together, the results support the involvement of both junctional communication 
      and secreted factor(s) in the propagation of radiation-induced stress to 
      bystander cells. They highlight the important role of radiation quality and dose 
      in the observed effects.
FAU - Autsavapromporn, Narongchai
AU  - Autsavapromporn N
AD  - a Research Center for Charged Particle Therapy, National Institute of 
      Radiological Sciences, Chiba, 263-8555, Japan.
FAU - Suzuki, Masao
AU  - Suzuki M
FAU - Funayama, Tomoo
AU  - Funayama T
FAU - Usami, Noriko
AU  - Usami N
FAU - Plante, Ianik
AU  - Plante I
FAU - Yokota, Yuichiro
AU  - Yokota Y
FAU - Mutou, Yasuko
AU  - Mutou Y
FAU - Ikeda, Hiroko
AU  - Ikeda H
FAU - Kobayashi, Katsumi
AU  - Kobayashi K
FAU - Kobayashi, Yasuhiko
AU  - Kobayashi Y
FAU - Uchihori, Yukio
AU  - Uchihori Y
FAU - Hei, Tom K
AU  - Hei TK
FAU - Azzam, Edouard I
AU  - Azzam EI
FAU - Murakami, Takeshi
AU  - Murakami T
LA  - eng
GR  - P01 CA049062/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130829
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Bystander Effect/*radiation effects
MH  - Cells, Cultured
MH  - DNA Damage
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/*cytology/*radiation effects
MH  - Gap Junctions/*radiation effects
MH  - Humans
MH  - Linear Energy Transfer
MH  - Monte Carlo Method
PMC - PMC4058832
MID - NIHMS584046
EDAT- 2013/08/31 06:00
MHDA- 2013/12/18 06:00
PMCR- 2014/06/16
CRDT- 2013/08/31 06:00
PHST- 2013/08/31 06:00 [entrez]
PHST- 2013/08/31 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PHST- 2014/06/16 00:00 [pmc-release]
AID - 10.1667/RR3111.1 [doi]
PST - ppublish
SO  - Radiat Res. 2013 Oct;180(4):367-75. doi: 10.1667/RR3111.1. Epub 2013 Aug 29.