PMID- 23990510
OWN - NLM
STAT- MEDLINE
DCOM- 20140709
LR  - 20220408
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 54
IP  - 4
DP  - 2013 Nov
TI  - Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) 
      and between H. vulgare and H. chilense using three-color fluorescence in situ 
      hybridization (FISH).
PG  - 427-33
LID - 10.1007/s13353-013-0167-8 [doi]
AB  - The aim of the present work was to study chromosomal polymorphism within 
      cultivated barley (Hordeum vulgare ssp. vulgare) using three-color fluorescence 
      in situ hybridization (FISH). The physical distribution of the most frequently 
      used, highly repetitive DNA sequences (GAA)7 specific for pericentromeric 
      heterochromatic regions, the ribosomal DNA clone pTa71, specific for the 45S 
      rDNA, and the barley-specific telomere-associated sequence HvT01, was 
      investigated to reveal genetic diversity in metaphase spreads of ten barley 
      genotypes with diverse geographical origin, growth habit and row number. A wild 
      relative of barley, Hordeum chilense was also studied in order to compare the 
      polymorphism between and within Hordeum species. Significant differences in the 
      hybridization patterns of all three DNA probes could be detected between the two 
      related species, but only probes pTa71 and HvT01 showed variation in the 
      intensity and/or position of hybridization sites among genotypes of H. vulgare 
      ssp. vulgare. The extent of polymorphism was less than that earlier reported for 
      molecular markers and was restricted to the long chromosome arms, with 
      differences between the chromosomes. 1H and 3H proved to be the most variable 
      chromosomes and 4H and 6H the most conserved.
FAU - Szakacs, E
AU  - Szakacs E
AD  - Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of 
      Sciences, 2462, Martonvasar, POB 19, Hungary, szakacse61@gmail.com.
FAU - Kruppa, K
AU  - Kruppa K
FAU - Molnar-Lang, M
AU  - Molnar-Lang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130829
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Chromosomes, Plant/*genetics
MH  - DNA Probes/genetics
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Genotype
MH  - Hordeum/classification/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Polymorphism, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2013/08/31 06:00
MHDA- 2014/07/10 06:00
CRDT- 2013/08/31 06:00
PHST- 2013/05/27 00:00 [received]
PHST- 2013/08/05 00:00 [accepted]
PHST- 2013/07/31 00:00 [revised]
PHST- 2013/08/31 06:00 [entrez]
PHST- 2013/08/31 06:00 [pubmed]
PHST- 2014/07/10 06:00 [medline]
AID - 10.1007/s13353-013-0167-8 [doi]
PST - ppublish
SO  - J Appl Genet. 2013 Nov;54(4):427-33. doi: 10.1007/s13353-013-0167-8. Epub 2013 
      Aug 29.