PMID- 23991222
OWN - NLM
STAT- MEDLINE
DCOM- 20140615
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 8
DP  - 2013
TI  - Isolation and characterization of a novel strain of mesenchymal stem cells from 
      mouse umbilical cord: potential application in cell-based therapy.
PG  - e74478
LID - 10.1371/journal.pone.0074478 [doi]
LID - e74478
AB  - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have recently been 
      recognized as a potential source for cell-based therapy in various preclinical 
      animal models, such as Parkinson's disease, cerebral ischemia, spinal cord 
      injury, and liver failure; however, the precise cellular and molecular mechanisms 
      underlying the beneficial outcomes remain under investigation. There is a growing 
      concern regarding rejection and alteration of genetic code using this 
      xenotransplantation approach. In this study, a novel strain of murine MSCs 
      derived from the umbilical cord of wild-type and green fluorescent protein (GFP) 
      transgenic mice have been successfully isolated, expanded, and characterized. 
      After 10 passages, the mUC-MSCs developed a rather homogeneous, triangular, 
      spindle-shaped morphology, and were sub-cultured up to 7 months (over 50 
      passages) without overt changes in morphology and doubling time. Cell surface 
      markers are quite similar to MSCs isolated from other tissue origins as well as 
      hUC-MSCs. These mUC-MSCs can differentiate into osteoblasts, adipocytes, neurons, 
      and astrocytes in vitro, as well as hematopoietic lineage cells in vivo. mUC-MSCs 
      also possess therapeutic potential against two disease models, focal ischemic 
      stroke induced by middle cerebral artery occlusion (MCAo) and acute hepatic 
      failure. Subtle differences in the expression of cytokine-related genes exist 
      between mUC-MSCs and hUC-MSCs, which may retard and jeopardize the advance of 
      cell therapy. Allografts of these newly established mUC-MSCs into various mouse 
      disease models may deepen our insights into the development of more effective 
      cell therapy regimens.
FAU - Li, Wen-Wen
AU  - Li WW
AD  - Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 
      Taipei, Taiwan.
FAU - Wei, Yau-Huei
AU  - Wei YH
FAU - Li, Hung
AU  - Li H
FAU - Lai, Dar-Ming
AU  - Lai DM
FAU - Lin, Teng-Nan
AU  - Lin TN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130826
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Adipocytes/cytology
MH  - Animals
MH  - Cell Differentiation
MH  - *Cell- and Tissue-Based Therapy
MH  - Flow Cytometry
MH  - Green Fluorescent Proteins/genetics
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neurons/cytology
MH  - Osteoblasts/cytology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stroke/therapy
MH  - Umbilical Cord/*cytology
PMC - PMC3753309
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/08/31 06:00
MHDA- 2014/06/16 06:00
PMCR- 2013/08/26
CRDT- 2013/08/31 06:00
PHST- 2013/07/02 00:00 [received]
PHST- 2013/07/31 00:00 [accepted]
PHST- 2013/08/31 06:00 [entrez]
PHST- 2013/08/31 06:00 [pubmed]
PHST- 2014/06/16 06:00 [medline]
PHST- 2013/08/26 00:00 [pmc-release]
AID - PONE-D-13-27225 [pii]
AID - 10.1371/journal.pone.0074478 [doi]
PST - epublish
SO  - PLoS One. 2013 Aug 26;8(8):e74478. doi: 10.1371/journal.pone.0074478. eCollection 
      2013.