PMID- 23991971
OWN - NLM
STAT- MEDLINE
DCOM- 20140318
LR  - 20190606
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Linking)
VI  - 14
IP  - 7
DP  - 2013
TI  - Prediction of promiscuous epitopes in the e6 protein of three high risk human 
      papilloma viruses: a computational approach.
PG  - 4167-75
AB  - A najor current challenge and constraint in cervical cancer research is the 
      development of vaccines against human papilloma virus (HPV) epitopes. Although 
      many studies are done on epitope identification on HPVs, no computational work 
      has been carried out for high risk forms which are considered to cause cervical 
      cancer. Of all the high risk HPVs, HPV 16, HPV 18 and HPV 45 are responsible for 
      94% of cervical cancers in women worldwide. In this work, we computationally 
      predicted the promiscuous epitopes among the E6 proteins of high risk HPVs. We 
      identified the conserved residues, HLA class I, HLA class II and B-cell epitopes 
      along with their corresponding secondary structure conformations. We used 
      extremely precise bioinformatics tools like ClustalW2, MAPPP, NetMHC, EpiJen, 
      EpiTop 1.0, ABCpred, BCpred and PSIPred for achieving this task. Our study 
      identified specific regions 'FAFR(K)DL' followed by 'KLPD(Q)LCTEL' fragments 
      which proved to be promiscuous epitopes present in both human leukocyte antigen 
      (HLA) class I, class II molecules and B cells as well. These fragments also 
      follow every suitable character to be considered as promiscuous epitopes with 
      supporting evidences of previously reported experimental results. Thus, we 
      conclude that these regions should be considered as the important for design of 
      specific therapeutic vaccines for cervical cancer.
FAU - Subramanian, Nirmala
AU  - Subramanian N
AD  - Bioinformatics Division, School of Biosciences and Technology, VIT University, 
      Vellore, India. csudandiradoss@vit.ac.in
FAU - Chinnappan, Sudandiradoss
AU  - Chinnappan S
LA  - eng
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (E6 protein, Human papillomavirus type 18)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Computational Biology
MH  - DNA-Binding Proteins/*immunology
MH  - Epitopes/*immunology
MH  - Female
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Viral/*immunology
MH  - Papillomaviridae/*immunology
MH  - Repressor Proteins/*immunology
MH  - Sequence Homology, Amino Acid
EDAT- 2013/09/03 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/09/03 06:00
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 10.7314/apjcp.2013.14.7.4167 [doi]
PST - ppublish
SO  - Asian Pac J Cancer Prev. 2013;14(7):4167-75. doi: 10.7314/apjcp.2013.14.7.4167.