PMID- 23993488
OWN - NLM
STAT- MEDLINE
DCOM- 20140328
LR  - 20130902
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 141
IP  - 4
DP  - 2013 Dec 15
TI  - Characterisation of genes encoding key enzymes involved in sugar metabolism of 
      apple fruit in controlled atmosphere storage.
PG  - 3323-8
LID - S0308-8146(13)00800-5 [pii]
LID - 10.1016/j.foodchem.2013.06.025 [doi]
AB  - Sugars are essential contributors to fruit flavour. Controlled atmosphere (CA) 
      storage has been proved to be beneficial for maintaining harvested fruit quality. 
      To explore regulatory mechanism of sugar metabolism in fruit stored in CA 
      condition, we cloned several genes, encoding key enzymes, involved in sugar 
      metabolism in apple fruit, and analyzed sugar contents, along with gene 
      expression and enzyme activities in fruits stored in air and CA. The results 
      indicated that CA could maintain higher contents of sugars, including sucrose, 
      fructose and glucose. Expression levels of key genes, such as sucrose synthase 
      (SS), sucrose phosphate synthase (SPS), fructokinase (FK) and hexokinase (HK), 
      were shown to be correlated with the corresponding enzyme activities. We found 
      that activities of neutral invertase (NI), vacuolar invertase (VI), FK and HK 
      were inhibited, but SPS activity was promoted in apple fruit stored in CA, 
      suggesting that CA storage could enhance sucrose synthesis and delay hydrolysis 
      of sucrose and hexose. These findings provided molecular evidence to explain why 
      higher sugar levels in harvested fruit are maintained under CA storage.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Zhu, Zhu
AU  - Zhu Z
AD  - Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of 
      Sciences, Beijing 100093, China.
FAU - Liu, Ruiling
AU  - Liu R
FAU - Li, Boqiang
AU  - Li B
FAU - Tian, Shiping
AU  - Tian S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130614
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Disaccharides)
RN  - 0 (Monosaccharides)
RN  - 0 (Plant Proteins)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - EC 2.4.1.13 (sucrose synthase)
RN  - EC 2.4.1.14 (sucrose-phosphate synthase)
RN  - EC 3.2.1.26 (beta-Fructofuranosidase)
SB  - IM
MH  - Cloning, Molecular
MH  - Disaccharides/*metabolism
MH  - Food Storage
MH  - Fruit/chemistry/*enzymology/genetics/metabolism
MH  - Glucosyltransferases/genetics/metabolism
MH  - Malus/chemistry/enzymology/*genetics/metabolism
MH  - Monosaccharides/*metabolism
MH  - Plant Proteins/*genetics/metabolism
MH  - beta-Fructofuranosidase/genetics/metabolism
OTO - NOTNLM
OT  - Apple fruit
OT  - Controlled atmosphere
OT  - Key genes
OT  - Sugar metabolism
EDAT- 2013/09/03 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/09/03 06:00
PHST- 2012/12/14 00:00 [received]
PHST- 2013/04/28 00:00 [revised]
PHST- 2013/06/06 00:00 [accepted]
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - S0308-8146(13)00800-5 [pii]
AID - 10.1016/j.foodchem.2013.06.025 [doi]
PST - ppublish
SO  - Food Chem. 2013 Dec 15;141(4):3323-8. doi: 10.1016/j.foodchem.2013.06.025. Epub 
      2013 Jun 14.