PMID- 23994062
OWN - NLM
STAT- MEDLINE
DCOM- 20140508
LR  - 20131007
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 553
DP  - 2013 Oct 11
TI  - Expression of neuropeptide Y and agouti-related protein mRNA stimulated by 
      glucocorticoids is attenuated via NF-kappaB p65 under ER stress in mouse hypothalamic 
      cultures.
PG  - 165-9
LID - S0304-3940(13)00773-8 [pii]
LID - 10.1016/j.neulet.2013.08.040 [doi]
AB  - There are several lines of evidence suggesting that glucocorticoid signaling in 
      the hypothalamus plays an important role in energy balance, and recent studies 
      suggest that endoplasmic reticulum (ER) stress in the hypothalamus could affect 
      signaling related to energy balance. In the present study, we examined the 
      regulation of glucocorticoid signaling under ER stress in mouse hypothalamic 
      organotypic cultures. Incubation of the hypothalamic explants with dexamethasone 
      (DEX) significantly increased expression levels of neuropeptide Y (NPY) and 
      agouti-related protein (AgRP) mRNA, and treatment with thapsigargin (TG), an ER 
      stressor, significantly attenuated DEX-induced NPY and AgRP mRNA expression. TG 
      treatment increased the levels of phospho-NF-kappaB p65 in hypothalamic cultures, and 
      inhibitors of NF-kappaB p65 reversed the inhibitory effects of TG on NPY and AgRP 
      expression. Our data thus demonstrated that glucocorticoid-stimulated NPY and 
      AgRP expression was attenuated via NF-kappaB p65 pathways under ER stress, and 
      suggest crosstalk between ER stress and inflammation in the hypothalamus.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Hagimoto, Shigeru
AU  - Hagimoto S
AD  - Department of Endocrinology and Diabetes, Nagoya University Graduate School of 
      Medicine, Nagoya 466-8550, Japan.
FAU - Arima, Hiroshi
AU  - Arima H
FAU - Adachi, Koichi
AU  - Adachi K
FAU - Ito, Yoshihiro
AU  - Ito Y
FAU - Suga, Hidetaka
AU  - Suga H
FAU - Sugimura, Yoshihisa
AU  - Sugimura Y
FAU - Goto, Motomitsu
AU  - Goto M
FAU - Banno, Ryoichi
AU  - Banno R
FAU - Oiso, Yutaka
AU  - Oiso Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130828
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Agouti-Related Protein)
RN  - 0 (Glucocorticoids)
RN  - 0 (Neuropeptide Y)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor RelA)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Agouti-Related Protein/genetics/*metabolism
MH  - Animals
MH  - Dexamethasone/pharmacology
MH  - *Endoplasmic Reticulum Stress
MH  - Glucocorticoids/pharmacology/*physiology
MH  - Hypothalamus/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuropeptide Y/genetics/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Signal Transduction
MH  - Thapsigargin/pharmacology
MH  - Tissue Culture Techniques
MH  - Transcription Factor RelA/*metabolism
OTO - NOTNLM
OT  - AgRP
OT  - Agouti-related proteins
OT  - DEX
OT  - ER
OT  - Endoplasmic reticulum stress
OT  - GR
OT  - Glucocorticoids
OT  - NF-kappaB
OT  - NPY
OT  - Neuropeptide Y
OT  - TG
OT  - TN
OT  - UPR
OT  - agouti-related protein
OT  - dexamethasone
OT  - endoplasmic reticulum
OT  - glucocorticoid receptor
OT  - neuropeptide Y
OT  - p38 MAPK
OT  - p38 mitogen-activated protein kinases
OT  - thapsigargin
OT  - tunicamycin
OT  - unfolded protein response
EDAT- 2013/09/03 06:00
MHDA- 2014/05/09 06:00
CRDT- 2013/09/03 06:00
PHST- 2013/06/08 00:00 [received]
PHST- 2013/08/07 00:00 [revised]
PHST- 2013/08/20 00:00 [accepted]
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2014/05/09 06:00 [medline]
AID - S0304-3940(13)00773-8 [pii]
AID - 10.1016/j.neulet.2013.08.040 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 Oct 11;553:165-9. doi: 10.1016/j.neulet.2013.08.040. Epub 
      2013 Aug 28.