PMID- 23996706
OWN - NLM
STAT- MEDLINE
DCOM- 20140424
LR  - 20130902
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 12
IP  - 4
DP  - 2013 Aug 28
TI  - Effect of heat and enzymatic treatments on human IgE and rabbit IgG sensitivity 
      to white bean allergens.
PG  - 304-11
AB  - The aim of this study was to evaluate the sensitivity of the population of Fez 
      and Casablanca in Morocco to dry white beans (Phaseolus Vulgaris) and to 
      investigate the effect of food processing (heat and/or enzymatic hydrolysis by 
      pepsin) on this sensitivity. Work was based on a bank consisting of 146 sera from 
      patients with atopic hypersensitivity in order to evaluate specific 
      immunoglobulin E (IgE) levels to native and processed white bean proteins by 
      ELISA. Under the same conditions, we assessed the immunoreactivity of rabbit IgG 
      obtained by immunization with native white bean proteins.Evaluation of specific 
      IgE to the white bean proteins showed that 51% of children and 39% of adults had 
      positive values. The heat treatment and pepsin hydrolysis of dry bean proteins 
      showed a reduction of 68% of IgE binding recognition in more than 65% of all 
      patients. After heating, all patients indicated a reduction greater than 36%. 
      With rabbit IgG, we observed a decrease by 25% of binding under heat treatment 
      while enzymatic digestion reduced IgG recognition by 46.6%.These findings suggest 
      that epitopes recognized by IgE in the studied population were conformational 
      sites whereas those recognized by rabbit IgG were probably sequential. In 
      conclusion, these results demonstrate that the Moroccan population was very 
      sensitive to white beans and this sensitivity could be reduced after heat 
      treatment or enzymatic hydrolysis.
FAU - Bousfiha, Amal
AU  - Bousfiha A
AD  - Laboratory of Bioactive Molecules, Faculty of Sciences and Techniques, Fez, 
      Morocco. amalben3@yahoo.fr.
FAU - Lotfi, Aarab
AU  - Lotfi A
LA  - eng
PT  - Journal Article
DEP - 20130828
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - Child
MH  - Child, Preschool
MH  - Electrophoresis
MH  - Fabaceae/*immunology
MH  - Food Hypersensitivity/*immunology/*therapy
MH  - *Hot Temperature
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Immunoglobulin G/*blood
MH  - Infant
MH  - Pepsin A/*pharmacology
MH  - Rabbits
EDAT- 2013/09/03 06:00
MHDA- 2014/04/25 06:00
CRDT- 2013/09/03 06:00
PHST- 2012/11/02 00:00 [received]
PHST- 2013/01/30 00:00 [accepted]
PHST- 2013/01/13 00:00 [revised]
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2014/04/25 06:00 [medline]
PST - epublish
SO  - Iran J Allergy Asthma Immunol. 2013 Aug 28;12(4):304-11.