PMID- 23996912
OWN - NLM
STAT- MEDLINE
DCOM- 20140513
LR  - 20191210
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 13
IP  - 10
DP  - 2013 Oct
TI  - Glycol chitosan-based nanogel as a potential targetable carrier for siRNA.
PG  - 1369-78
LID - 10.1002/mabi.201300123 [doi]
AB  - A self-assembled glycol chitosan nanogel (GC) is synthesized by chemically 
      grafting hydrophobic chains onto a polysaccharide, which is comprehensively 
      characterized. The obtained macromolecular micelle is decorated with 
      folate-conjugated poly(ethylene glycol) (PEG) (GCFA). An average size 
      distribution of 250 and 200 nm is observed, respectively for the GC and GCFA 
      nanogels. Differential cell localization is observed on incubating the materials 
      with HeLa cells. Whereas the GC nanogel is detected on the cell surface, GCFA is 
      localized in the cytoplasm. The cell viability is not compromised by the 
      nanogels. Interestingly, GC nanogel is poorly internalized by bone marrow derived 
      macrophages (BMDMs), and GCFA is not phagocytosed. Given its ability to complex 
      siRNA, the targetable GC nanogel can be a promising vehicle for siRNA delivery.
CI  - (c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Pereira, Paula
AU  - Pereira P
AD  - Institute for Biotechnology and Bioengineering (IBB), Centre for Biological 
      Engineering, Campus de Gualtar, University of Minho, Braga, Portugal.
FAU - Morgado, Daniela
AU  - Morgado D
FAU - Crepet, Agnes
AU  - Crepet A
FAU - David, Laurent
AU  - David L
FAU - Gama, Francisco M
AU  - Gama FM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130828
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Drug Carriers)
RN  - 0 (Nanogels)
RN  - 0 (Polymers)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (glycol-chitosan)
RN  - 0 (polyethylene glycol polyethyleneimine nanogel)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9012-76-4 (Chitosan)
SB  - IM
EIN - Macromol Biosci. 2014 Apr;14(4):597
MH  - Cell Survival/drug effects
MH  - Chitosan/*administration & dosage/chemical synthesis/chemistry
MH  - Drug Carriers/administration & dosage/chemical synthesis/chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - *Molecular Targeted Therapy
MH  - Nanogels
MH  - Polyethylene Glycols/*administration & dosage/chemical synthesis/chemistry
MH  - Polyethyleneimine/*administration & dosage/chemical synthesis/chemistry
MH  - Polymers/chemical synthesis/chemistry
MH  - RNA, Small Interfering/*administration & dosage/genetics
OTO - NOTNLM
OT  - cellular uptake and siRNA delivery
OT  - cytotoxicity
OT  - glycol chitosan nanogel
EDAT- 2013/09/03 06:00
MHDA- 2014/05/14 06:00
CRDT- 2013/09/03 06:00
PHST- 2013/02/28 00:00 [received]
PHST- 2013/05/24 00:00 [revised]
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2014/05/14 06:00 [medline]
AID - 10.1002/mabi.201300123 [doi]
PST - ppublish
SO  - Macromol Biosci. 2013 Oct;13(10):1369-78. doi: 10.1002/mabi.201300123. Epub 2013 
      Aug 28.