PMID- 23997019
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20200930
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 102
IP  - 1
DP  - 2014 Jan
TI  - In vitro macrophage response to nanometer-size chromium oxide particles.
PG  - 149-59
LID - 10.1002/jbm.b.32991 [doi]
AB  - An increasing number of studies have reported adverse tissue reactions around 
      metal-on-metal (MM) hip implants. However, the origin and mechanisms of these 
      reactions remain unclear. Moreover, the biological effects of nanometer-size 
      chromium oxide particles, the predominant type of wear particles produced by MM 
      implants, remain mostly unknown. The purpose of this study was to analyze the 
      cytotoxic effects of clinically relevant nanometer-size chromium oxide particles 
      on macrophage response in vitro. J774.A1 macrophages were cultured with either 60 
      nm or 700 nm commercially available Cr2 O3 particles at different concentrations. 
      Two different particle sizes were analyzed to evaluate potential volume effects. 
      Cell mortality was analyzed by light microscopy, flow cytometry (annexin 
      V-fluorescein isothiocyanate and propidium iodide assay), and using a cell death 
      detection enzyme-linked immunosorbant assay (ELISA). Tumor necrosis factor alpha 
      (TNF-alpha), monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory 
      protein-1 alpha (MIP-1alpha) release was measured by ELISA, and gene expression was 
      analyzed by quantitative real-time PCR. Results showed that, at high 
      concentrations, Cr2 O3 particles of both sizes can be cytotoxic, inducing 
      significant decreases in total cell numbers and increases in necrosis. Results 
      also suggested that these effects were dependent on particle volume. However, 
      TNF-alpha, MCP-1, and MIP-1alpha cytokine release and gene expression remained low. 
      Overall, this study demonstrates that nanometer-size particles of Cr2 O3 , a 
      stable form of chromium oxide ceramic, have rather low cytotoxic effects on 
      macrophages. Therefore, these particles may not be the main culprit in the 
      initiation of the inflammatory reaction in MM periprosthetic tissues. However, 
      other parameters (e.g., potential intracellular damage) remain to be 
      investigated.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - VanOs, Robilyn
AU  - VanOs R
AD  - Department of Mechanical Engineering, University of Ottawa, Ottawa, Ontario K1N 
      6N5, Canada.
FAU - Lildhar, Levannia L
AU  - Lildhar LL
FAU - Lehoux, Eric A
AU  - Lehoux EA
FAU - Beaule, Paul E
AU  - Beaule PE
FAU - Catelas, Isabelle
AU  - Catelas I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130829
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccl3 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chromium Compounds)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - X5Z09SU859 (chromic oxide)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Biocompatible Materials/chemistry/*toxicity
MH  - Cell Line
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokine CCL3/genetics/metabolism
MH  - Chromium Compounds/chemistry/*toxicity
MH  - Equipment Failure Analysis
MH  - Gene Expression/drug effects
MH  - Hip Prosthesis/adverse effects
MH  - Humans
MH  - Macrophage Activation/*drug effects/physiology
MH  - Macrophages/drug effects/pathology/physiology
MH  - Materials Testing
MH  - Metal Nanoparticles/chemistry/*toxicity/ultrastructure
MH  - Mice
MH  - Necrosis
MH  - Particle Size
MH  - Prosthesis Failure
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
OTO - NOTNLM
OT  - chromium oxide
OT  - cytotoxicity
OT  - hip implants
OT  - macrophages
OT  - wear particles
EDAT- 2013/09/03 06:00
MHDA- 2015/05/13 06:00
CRDT- 2013/09/03 06:00
PHST- 2012/10/22 00:00 [received]
PHST- 2013/04/05 00:00 [revised]
PHST- 2013/05/28 00:00 [accepted]
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - 10.1002/jbm.b.32991 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2014 Jan;102(1):149-59. doi: 
      10.1002/jbm.b.32991. Epub 2013 Aug 29.