PMID- 23997098
OWN - NLM
STAT- MEDLINE
DCOM- 20131227
LR  - 20211021
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Print)
IS  - 0363-6135 (Linking)
VI  - 305
IP  - 9
DP  - 2013 Nov 1
TI  - Identification of small juvenile stem cells in aged bone marrow and their 
      therapeutic potential for repair of the ischemic heart.
PG  - H1354-62
LID - 10.1152/ajpheart.00379.2013 [doi]
AB  - Stem cell-mediated cardiac regeneration is impaired with age. In this study, we 
      identified a novel subpopulation of small juvenile stem cells (SJSCs) isolated 
      from aged bone marrow-derived stem cells (BMSCs) with high proliferation and 
      differentiation potential. SJSCs expressed mesenchymal stem cell markers, 
      CD29(+)/CD44(+)/CD59(+)/CD90(+), but were negative for CD45(-)/CD117(-) as 
      examined by flow cytometry analysis. SJSCs showed higher proliferation, colony 
      formation, and differentiation abilities compared with BMSCs. We also observed 
      that SJSCs significantly expressed cardiac lineage markers (Gata-4 and 
      myocyte-specific enhancer factor 2C) and pluripotency markers (octamer-binding 
      transcription factor 4, sex-determining region Y box 2, stage-specific embryonic 
      antigen 1, and Nanog) as well as antiaging factors such as telomerase reverse 
      transcriptase and sirtuin 1. Interestingly, SJSCs either from young or aged 
      animals showed significantly longer telomere length as well as lower 
      senescence-associated beta-galactosidase expression, suggesting that SJSCs possess 
      antiaging properties, whereas aged BMSCs have limited potential for proliferation 
      and differentiation. Furthermore, transplantation of aged SJSCs into the 
      infarcted rat heart significantly reduced the infarction size and improved left 
      ventricular function, whereas transplantation of aged BMSCs was less effective. 
      Moreover, neovascularization as well as cardiomyogenic differentiation in the 
      peri-infarcted area were significantly increased in the SJSC-transplanted group 
      compared with the BMSC-transplated group, as evaluated by immunohistochemical 
      analysis. Taken together, these findings demonstrate that SJSCs possess 
      characteristics of antiaging, pluripotency, and high proliferation and 
      differentiation rates, and, therefore, these cells offer great therapeutic 
      potential for repair of the injured myocardium.
FAU - Igura, Koichi
AU  - Igura K
AD  - Department of Pathology and Lab of Medicine, University of Cincinnati Medical 
      Center, Cincinnati, Ohio.
FAU - Okada, Motoi
AU  - Okada M
FAU - Kim, Ha Won
AU  - Kim HW
FAU - Ashraf, Muhammad
AU  - Ashraf M
LA  - eng
GR  - HL-087246/HL/NHLBI NIH HHS/United States
GR  - HL-095375/HL/NHLBI NIH HHS/United States
GR  - R37-HL-074272/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130830
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 0 (Biomarkers)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Biomarkers/metabolism
MH  - *Bone Marrow Cells/metabolism
MH  - *Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - *Cellular Senescence
MH  - Disease Models, Animal
MH  - Female
MH  - Myocardial Infarction/metabolism/pathology/physiopathology/*surgery
MH  - Myocardium/metabolism/*pathology
MH  - Pluripotent Stem Cells/metabolism/*transplantation
MH  - Rats
MH  - Rats, Inbred F344
MH  - Recovery of Function
MH  - *Regeneration
MH  - Telomere/metabolism
MH  - Telomere Homeostasis
MH  - Time Factors
MH  - Ventricular Function, Left
PMC - PMC3840241
OTO - NOTNLM
OT  - antiaging
OT  - cardiac repair
OT  - small juvenile stem cell
EDAT- 2013/09/03 06:00
MHDA- 2013/12/29 06:00
PMCR- 2014/11/01
CRDT- 2013/09/03 06:00
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2013/12/29 06:00 [medline]
PHST- 2014/11/01 00:00 [pmc-release]
AID - ajpheart.00379.2013 [pii]
AID - H-00379-2013 [pii]
AID - 10.1152/ajpheart.00379.2013 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2013 Nov 1;305(9):H1354-62. doi: 
      10.1152/ajpheart.00379.2013. Epub 2013 Aug 30.