PMID- 23997757
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240420
IS  - 1573-3882 (Print)
IS  - 1573-3890 (Electronic)
IS  - 1573-3882 (Linking)
VI  - 9
IP  - 4
DP  - 2013 Aug 1
TI  - A targeted metabolomic protocol for short-chain fatty acids and branched-chain 
      amino acids.
PG  - 818-827
AB  - Research in obesity and metabolic disorders that involve intestinal microbiota 
      demands reliable methods for the precise measurement of the short-chain fatty 
      acids (SCFAs) and branched-chain amino acids (BCAAs) concentration. Here, we 
      report a rapid method of simultaneously determining SCFAs and BCAAs in biological 
      samples using propyl chloroformate (PCF) derivatization followed by gas 
      chromatography mass spectrometry (GC-MS) analysis. A one-step derivatization 
      using 100 microL of PCF in a reaction system of water, propanol, and pyridine (v/v/v 
      = 8:3:2) at pH 8 provided the optimal derivatization efficiency. The best 
      extraction efficiency of the derivatized products was achieved by a two-step 
      extraction with hexane. The method exhibited good derivatization efficiency and 
      recovery for a wide range of concentrations with a low limit of detection for 
      each compound. The relative standard deviations (RSDs) of all targeted compounds 
      showed good intra- and inter-day (within 7 days) precision (< 10%), and good 
      stability (< 20%) within 4 days at room temperature (23-25  degrees C), or 7 days when 
      stored at -20  degrees C. We applied our method to measure SCFA and BCAA levels in fecal 
      samples from rats administrated with different diet. Both univariate and 
      multivariate statistics analysis of the concentrations of these target 
      metabolites could differentiate three groups with ethanol intervention and 
      different oils in diet. This method was also successfully employed to determine 
      SCFA and BCAA in the feces, plasma and urine from normal humans, providing 
      important baseline information of the concentrations of these metabolites. This 
      novel metabolic profile study has great potential for translational research.
FAU - Zheng, Xiaojiao
AU  - Zheng X
AD  - Center for Translational Medicine, and Shanghai Key Laboratory of Diabetes 
      Mellitus, Department of Endocrinology and Metabolism, Shanghai Jiao Tong 
      University Affiliated Sixth People's Hospital, Shanghai 200233, China.
FAU - Qiu, Yunping
AU  - Qiu Y
FAU - Zhong, Wei
AU  - Zhong W
FAU - Baxter, Sarah
AU  - Baxter S
FAU - Su, Mingming
AU  - Su M
FAU - Li, Qiong
AU  - Li Q
FAU - Xie, Guoxiang
AU  - Xie G
FAU - Ore, Brandon M
AU  - Ore BM
FAU - Qiao, Shanlei
AU  - Qiao S
FAU - Spencer, Melanie D
AU  - Spencer MD
FAU - Zeisel, Steven H
AU  - Zeisel SH
FAU - Zhou, Zhanxiang
AU  - Zhou Z
FAU - Zhao, Aihua
AU  - Zhao A
FAU - Jia, Wei
AU  - Jia W
LA  - eng
GR  - P30 DK056350/DK/NIDDK NIH HHS/United States
GR  - R01 AA020212/AA/NIAAA NIH HHS/United States
GR  - T32 DK007737/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Metabolomics
JT  - Metabolomics : Official journal of the Metabolomic Society
JID - 101274889
PMC - PMC3756605
MID - NIHMS441818
OTO - NOTNLM
OT  - Biological samples
OT  - Branched-chain amino acids
OT  - Gas chromatography/mass spectrometry
OT  - Propyl chloroformate
OT  - Short-chain fatty acids
OT  - Targeted metabolomics
EDAT- 2013/09/03 06:00
MHDA- 2013/09/03 06:01
PMCR- 2014/08/01
CRDT- 2013/09/03 06:00
PHST- 2013/09/03 06:00 [entrez]
PHST- 2013/09/03 06:00 [pubmed]
PHST- 2013/09/03 06:01 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1007/s11306-013-0500-6 [doi]
PST - ppublish
SO  - Metabolomics. 2013 Aug 1;9(4):818-827. doi: 10.1007/s11306-013-0500-6.