PMID- 23999049
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20220311
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 41
IP  - 4
DP  - 2013 Dec
TI  - Molecular and functional characterization of IL-1 receptor type 2 in grass carp: 
      a potent inhibitor of IL-1beta signaling in head kidney leukocytes.
PG  - 738-45
LID - S0145-305X(13)00250-4 [pii]
LID - 10.1016/j.dci.2013.08.023 [doi]
AB  - IL-1 receptor type 2 (IL-1R2) is known as one of natural IL-1beta singling 
      inhibitors in mammals. However, the functional role of IL-1R2 in fish remains 
      largely unknown. In this study, grass carp (Ctenopharyngodon idellus) IL-1R2 
      (gcIL-1R2) was identified and functionally characterized. Similar to its fish 
      homologs, the deduced protein of gcIL-1R2 possessed two Ig-like domains in its 
      extracellular region but lacked an intracellular signaling domain. The 
      involvement of gcIL-1R2 in immune response was demonstrated by investigating its 
      expression profiles in head kidney and head kidney leukocytes (HKLs) following in 
      vivo bacterial infection and in vitro LPS treatment, respectively. Moreover, 
      recombinant grass carp IL-1beta (rgcIL-1beta) was able to stimulate gcIL-1R2 mRNA 
      expression with a rapid kinetics. This stimulation was possibly dependent on p38, 
      JNK, p42/44 and NF-kappaB pathways in grass carp HKLs, revealing a new regulatory 
      point of IL-1beta signaling at receptor level in fish. Furthermore, recombinant 
      protein of the gcIL-1R2 extracellular region (rgcIL-1R2) was demonstrated to 
      interact with rgcIL-1beta by using ELISA, elucidating the binding specificity of 
      gcIL-1R2. Importantly, the stimulatory effect of rgcIL-1beta on its own mRNA 
      expression was blocked by rgcIL-1R2 in a dose-dependent manner in grass carp 
      HKLs, providing the evidence for a functional role of IL-1R2 in IL-1beta signaling 
      in teleost. These findings suggested that teleost IL-1R2 may serve as a local 
      naturally occurring inhibitor involving in IL-1beta signaling as seen in mammals.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Yang, Xiao
AU  - Yang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu 610054, People's Republic of China.
FAU - Wang, Shangnian
AU  - Wang S
FAU - Du, Linyong
AU  - Du L
FAU - Yang, Kun
AU  - Yang K
FAU - Wang, Xinyan
AU  - Wang X
FAU - Zhang, Anying
AU  - Zhang A
FAU - Zhou, Hong
AU  - Zhou H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130830
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-1 Type II)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Infections/genetics/immunology/metabolism/microbiology
MH  - Base Sequence
MH  - Carps/genetics/immunology/*metabolism/microbiology
MH  - Cloning, Molecular/methods
MH  - DNA, Complementary/genetics
MH  - Head Kidney/immunology/*metabolism/microbiology
MH  - Interleukin-1beta/genetics/immunology/*metabolism
MH  - Leukocytes/immunology/*metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/genetics
MH  - Receptors, Interleukin-1 Type II/genetics/immunology/*metabolism
MH  - Recombinant Proteins/genetics/immunology/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - Grass carp
OT  - IL-1 receptor type 2
OT  - IL-1beta
OT  - Natural IL-1beta singling inhibitor
OT  - Receptor binding
EDAT- 2013/09/04 06:00
MHDA- 2014/06/24 06:00
CRDT- 2013/09/04 06:00
PHST- 2013/07/09 00:00 [received]
PHST- 2013/08/23 00:00 [revised]
PHST- 2013/08/24 00:00 [accepted]
PHST- 2013/09/04 06:00 [entrez]
PHST- 2013/09/04 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - S0145-305X(13)00250-4 [pii]
AID - 10.1016/j.dci.2013.08.023 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2013 Dec;41(4):738-45. doi: 10.1016/j.dci.2013.08.023. Epub 
      2013 Aug 30.