PMID- 23999070
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20131107
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Linking)
VI  - 74
IP  - 5
DP  - 2013 Nov
TI  - Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes 
      from newborn preterm and term infants.
PG  - 503-10
LID - 10.1038/pr.2013.145 [doi]
AB  - BACKGROUND: Deficiencies in phagocytosis may contribute to the increased 
      susceptibility of infants to early life infections. Data on phagocytosis of the 
      major neonatal pathogens Staphylococcus epidermidis (SE), Staphylococcus aureus 
      (SA), and Escherichia coli (EC) by preterm infant leukocytes are inconsistent. 
      METHODS: Cord and <24-h peripheral blood were collected from very preterm (<30.1 
      wks gestational age (GA)) and term (37-42 wks GA) infants. Monocyte and 
      neutrophil phagocytosis of pHrodo-labeled SE, SA, and EC were analyzed using a 
      small-volume flow cytometry assay, with simultaneous characterization of surface 
      activation marker expression. RESULTS: Preterm infants had lower proportions of 
      monocytes and neutrophils capable of phagocytosis than term infants, but preterm 
      infant phagocytes had higher phagocytic capacity. Phagocytosis was strongly 
      correlated between cord and <24-h peripheral blood. Supplementation with 
      exogenous complement significantly increased phagocytosis of EC but not of SE or 
      SA. Monocyte human leukocyte antigen (HLA)-DR expression was lower in preterm 
      infants but did not correlate with phagocytosis. CONCLUSION: There is no defect 
      in phagocytosis by monocytes and neutrophils from preterm compared with term 
      infants, although preterm infants possess fewer phagocytes, possibly contributing 
      to susceptibility to bacterial infection. Further investigation into the 
      development of postnatal phagocytic competence is warranted.
FAU - Prosser, Amy
AU  - Prosser A
AD  - School of Paediatrics and Child Health, University of Western Australia, Perth, 
      Western Australia, Australia.
FAU - Hibbert, Julie
AU  - Hibbert J
FAU - Strunk, Tobias
AU  - Strunk T
FAU - Kok, Chooi Heen
AU  - Kok CH
FAU - Simmer, Karen
AU  - Simmer K
FAU - Richmond, Peter
AU  - Richmond P
FAU - Burgner, David
AU  - Burgner D
FAU - Currie, Andrew
AU  - Currie A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130902
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
SB  - IM
MH  - Escherichia coli/immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Infant, Newborn
MH  - Infant, Premature/*immunology
MH  - Monocytes/*immunology
MH  - Neutrophils/*immunology
MH  - Phagocytosis/*immunology
MH  - Staphylococcus aureus/immunology
MH  - Staphylococcus epidermidis/immunology
MH  - Statistics, Nonparametric
EDAT- 2013/09/04 06:00
MHDA- 2014/06/17 06:00
CRDT- 2013/09/04 06:00
PHST- 2012/06/21 00:00 [received]
PHST- 2013/03/27 00:00 [accepted]
PHST- 2013/09/04 06:00 [entrez]
PHST- 2013/09/04 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
AID - pr2013145 [pii]
AID - 10.1038/pr.2013.145 [doi]
PST - ppublish
SO  - Pediatr Res. 2013 Nov;74(5):503-10. doi: 10.1038/pr.2013.145. Epub 2013 Sep 2.