PMID- 23999075
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20211021
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 74
IP  - 5
DP  - 2013 Nov
TI  - Kidins220/ARMS is expressed in neuroblastoma tumors and stabilizes neurotrophic 
      signaling in a human neuroblastoma cell line.
PG  - 517-24
LID - 10.1038/pr.2013.146 [doi]
AB  - BACKGROUND: Neurotrophic signaling is an important factor in the survival of 
      developing neurons, and the expression of neurotrophic receptors correlates with 
      prognosis in neuroblastoma. Kinase D-interacting substrate of 220 kDa (Kidins220) 
      associates with neurotrophic receptors and stabilizes them, but the expression 
      and function of Kidins220 in neuroblastoma are unknown. METHODS: We study 
      Kidins220 expression in human neuroblastoma cell lines and tumor samples by 
      western blotting and microarray analyses. We determine the functional 
      consequences of downregulation of Kidins220 for response of cell lines to 
      oxidative stress, chemotherapeutic treatment, and neurotrophins using small 
      interfering RNA silencing and by measuring cell survival, signaling, and 
      migration. RESULTS: Kidins220 is expressed in all neuroblastoma tumors and cell 
      lines studied. Downregulation of Kidins220 leads to attenuation of nerve growth 
      factor (NGF)-induced, but not brain-derived neurotrophic factor (BDNF)-induced, 
      MAPK signaling. However, downregulation of Kidins220 does not alter the response 
      to chemotherapeutic drugs or oxidative stress or affect cellular motility. 
      CONCLUSION: Kidins220 is expressed in neuroblastoma tumors and stabilizes 
      NGF-induced, but not BDNF-induced, survival signaling in neuroblastoma cell 
      lines.
FAU - Rogers, Danny A
AU  - Rogers DA
AD  - 1] Department of Pediatrics, University of Rochester Medical Center, Rochester, 
      New York [2] Department of Neurology, University of Rochester Medical Center, 
      Rochester, New York [3] Department of Neurobiology and Anatomy, University of 
      Rochester Medical Center, Rochester, New York.
FAU - Schor, Nina F
AU  - Schor NF
LA  - eng
GR  - R01 CA074289/CA/NCI NIH HHS/United States
GR  - R01 NS038569/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130902
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (KIDINS220 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Survival
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Membrane Proteins/genetics/*metabolism
MH  - Microarray Analysis
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neuroblastoma/genetics/*metabolism
MH  - Oxidative Stress/genetics/physiology
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction/genetics/*physiology
PMC - PMC3968798
MID - NIHMS548702
EDAT- 2013/09/04 06:00
MHDA- 2014/06/17 06:00
PMCR- 2014/05/01
CRDT- 2013/09/04 06:00
PHST- 2012/12/02 00:00 [received]
PHST- 2013/05/21 00:00 [accepted]
PHST- 2013/09/04 06:00 [entrez]
PHST- 2013/09/04 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
PHST- 2014/05/01 00:00 [pmc-release]
AID - pr2013146 [pii]
AID - 10.1038/pr.2013.146 [doi]
PST - ppublish
SO  - Pediatr Res. 2013 Nov;74(5):517-24. doi: 10.1038/pr.2013.146. Epub 2013 Sep 2.