PMID- 24004240
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20220316
IS  - 1563-5279 (Electronic)
IS  - 0020-7454 (Linking)
VI  - 124
IP  - 3
DP  - 2014 Mar
TI  - Electroacupuncture enhances hippocampal NSCs proliferation in cerebral 
      ischemia-reperfusion injured rats via activation of notch signaling pathway.
PG  - 204-12
LID - 10.3109/00207454.2013.840781 [doi]
AB  - The purpose of this study was to investigate the mechanisms by which 
      electroacupuncture (EA) enhances hippocampal neural stem cells (NSCs) 
      proliferation in cerebral ischemia-reperfusion (I/R) injured rats. A total of 72 
      male adult Sprague-Dawley rats were randomly divided into the sham operation 
      control group (SC), the ischemia control group (IC) and the EA group. Middle 
      cerebral artery occlusion (MCAO) was performed to establish the focal cerebral 
      I/R injury model. Proliferation of hippocampal NSCs in cerebral I/R injured rats 
      was determined by the Nestin immunohistochemical staining. Activation of the 
      notch signaling pathway was detected by Western blotting and reverse 
      transcription polymerase chain reaction analysis. The serum level of neurotrophic 
      factors, e.g., the brain-derived neurotrophic factor (BDNF) and the Glial cell 
      line-derived neurotrophic factor (GDNF), was measured using enzyme-linked 
      immunosorbent assay (ELISA). The results showed that EA at Quchi (LI11) and 
      Zusanli (ST36) acupoints significantly alleviated neurological deficits, reduced 
      infarct volumes and promoted the proliferation of hippocampal NSCs in cerebral 
      I/R injured rats. The crucial signaling molecules in the notch signaling pathway 
      were activated and the secretion of BDNF and GDNF was increased upon EA. The 
      protein and mRNA levels of Cyclin D1, Cdk4 and p-Rb were increased, while p21 and 
      p27 transcripts were suppressed by notch signaling. These results suggest that 
      the up-regulatory effect of EA on the notch signaling pathway and neurotrophic 
      factor secretion may result in the promotion of NSCs proliferation and 
      consequently a therapeutic effect on cerebral ischemia.
FAU - Tao, Jing
AU  - Tao J
AD  - 1College of Rehabilitation Medicine.
FAU - Chen, Bin
AU  - Chen B
FAU - Gao, Yanling
AU  - Gao Y
FAU - Yang, Shanli
AU  - Yang S
FAU - Huang, Jia
AU  - Huang J
FAU - Jiang, Xin
AU  - Jiang X
FAU - Wu, Yayin
AU  - Wu Y
FAU - Peng, Jun
AU  - Peng J
FAU - Hong, Zhenfeng
AU  - Hong Z
FAU - Chen, Lidian
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130927
PL  - England
TA  - Int J Neurosci
JT  - The International journal of neuroscience
JID - 0270707
RN  - 0 (Nestin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Notch)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain Ischemia/*pathology/therapy
MH  - *Cell Proliferation
MH  - Disease Models, Animal
MH  - Electroacupuncture/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hippocampus/*cytology
MH  - Male
MH  - Nestin/metabolism
MH  - Neural Stem Cells/*physiology
MH  - Neurologic Examination
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Notch/*metabolism
MH  - Reperfusion Injury/*pathology
MH  - Signal Transduction/*physiology
EDAT- 2013/09/06 06:00
MHDA- 2014/09/26 06:00
CRDT- 2013/09/06 06:00
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
AID - 10.3109/00207454.2013.840781 [doi]
PST - ppublish
SO  - Int J Neurosci. 2014 Mar;124(3):204-12. doi: 10.3109/00207454.2013.840781. Epub 
      2013 Sep 27.