PMID- 24005192
OWN - NLM
STAT- MEDLINE
DCOM- 20140624
LR  - 20161125
IS  - 1938-9116 (Electronic)
IS  - 1538-5744 (Linking)
VI  - 47
IP  - 8
DP  - 2013 Nov
TI  - Intravascular radiocontrast iodixanol increases permeability of proximal tubule 
      epithelium: a possible mechanism of contrast-induced nephropathy.
PG  - 632-8
LID - 10.1177/1538574413503560 [doi]
AB  - PURPOSE: To investigate the effect of Iodixanol on kidney proximal tubular cell 
      line human kidney 2 (HK-2). METHODS: The HK-2 cells were treated with Iodixanol. 
      A Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay was 
      used to evaluate apoptosis. Cell viability was measured by proliferation assay 
      kit. Cell permeability changes were assessed by transwell assay and intercellular 
      gaps measurement. Expression of claudin-2 was assessed by quantitative reverse 
      transcriptase-polymerase chain reaction (qRT-PCR) and Western blot. RESULTS: 
      Iodixanol reduced tubule cell viability (P < .01) but did not cause apoptosis. 
      The intercellular gap formation (P < .01) and transwell (P < .05) assays revealed 
      that cell permeability significantly increased after Iodixanol treatment of 
      monolayer cells. Western blot and qRT-PCR showed significant upregulation of 
      claudin-2 protein (P < .05) and messenger RNA expression (P < .01). CONCLUSIONS: 
      Our in vitro data do not support the hypothesis that direct kidney cell death 
      from Iodixanol is a major mechanism of contrast-induced nephropathy (CIN). 
      However, increased permeability of proximal tubule epithelium caused by Iodixanol 
      may play an important role in CIN.
FAU - Yao, Luyu
AU  - Yao L
AD  - 1Department of Surgery, Louisiana State University Health Sciences Center, 
      Shreveport, LA, USA.
FAU - Kolluru, Gopi K
AU  - Kolluru GK
FAU - Kevil, Christopher G
AU  - Kevil CG
FAU - Zhang, Wayne W
AU  - Zhang WW
LA  - eng
PT  - Journal Article
DEP - 20130904
PL  - United States
TA  - Vasc Endovascular Surg
JT  - Vascular and endovascular surgery
JID - 101136421
RN  - 0 (CLDN2 protein, human)
RN  - 0 (Claudins)
RN  - 0 (Contrast Media)
RN  - 0 (Triiodobenzoic Acids)
RN  - HW8W27HTXX (iodixanol)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Claudins/genetics/metabolism
MH  - Contrast Media/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells/*drug effects/metabolism/pathology
MH  - Humans
MH  - Intercellular Junctions/*drug effects/metabolism/pathology
MH  - Kidney Tubules, Proximal/*drug effects/metabolism/pathology
MH  - Permeability
MH  - Triiodobenzoic Acids/*toxicity
MH  - Up-Regulation
OTO - NOTNLM
OT  - contrast-induced nephropathy
OT  - iodixanol
OT  - permeability
OT  - proximal tubule epithelium
EDAT- 2013/09/06 06:00
MHDA- 2014/06/25 06:00
CRDT- 2013/09/06 06:00
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/06/25 06:00 [medline]
AID - 1538574413503560 [pii]
AID - 10.1177/1538574413503560 [doi]
PST - ppublish
SO  - Vasc Endovascular Surg. 2013 Nov;47(8):632-8. doi: 10.1177/1538574413503560. Epub 
      2013 Sep 4.