PMID- 24005538
OWN - NLM
STAT- MEDLINE
DCOM- 20140907
LR  - 20211021
IS  - 1689-1392 (Electronic)
IS  - 1425-8153 (Print)
IS  - 1425-8153 (Linking)
VI  - 18
IP  - 4
DP  - 2013 Dec
TI  - In vitro and in vivo characteristics of hepatic oval cells modified with human 
      hepatocyte growth factor.
PG  - 507-21
LID - 10.2478/s11658-013-0104-1 [doi]
AB  - Hepatocyte growth factor (HGF) is a multifunctional growth factor that controls 
      cell scattering. It has been suggested that it regulates the proliferation of 
      hepatic oval cells (HOCs). Using a HOC line that stably expresses the human HGF 
      gene (hHGF), we investigated the in vitro proliferation and differentiation 
      characteristics of hHGF-modified HOCs and explored their potential capacity for 
      intrahepatic transplantation. A modified 2-acetylaminofluorene and partial 
      hepatectomy (2-AAF/PH) model was established to activate the proliferation of 
      oval cells in the rat liver. HOCs were transfected with the pBLAST2-hHGF plasmid 
      and hHGF-carrying HOCs were selected based on blasticidin resistance. The level 
      of hHGF secretion was determined via ELISA. Cell proliferation was determined 
      using the MTT assay. Differentiation was induced by growth factor withdrawal. A 
      two-cuff technique was used for orthotopic liver transplantation, and HOCs or 
      hHGF-modified HOCs were transplanted into the recipients. The levels of 
      biochemical indicators of liver function were measured after transplantation. An 
      HOC line stably expressing hHGF was established. The transfected line showed 
      greater hHGF secretion than normal HOCs. The hHGF gene promoted the proliferation 
      capability of HOCs by reducing the peak time in vitro. The hHGF-modified HOCs 
      differentiated into hepatocytes and bile duct epithelial cells upon growth factor 
      withdrawal in vitro. In addition, hHGF-modified HOC transplantation significantly 
      prolonged the median survival time (MST) and improved the liver function of 
      recipients compared to HOC transplant recipients and nontransplanted controls. 
      Our results indicate that hHGF-modified HOCs may have valuable properties for 
      therapeutic liver regeneration after orthotopic liver transplantation.
FAU - Li, Zhu
AU  - Li Z
AD  - Department of Hepatobiliary Surgery, Liaocheng People's Hospital, No. 67 
      Dongchang West Road, Liaocheng, 252000, Shandong, China, lllllzhu@126.com.
FAU - Chen, Juan
AU  - Chen J
FAU - Li, Li
AU  - Li L
FAU - Ran, Jiang-Hua
AU  - Ran JH
FAU - Li, Xue-Hua
AU  - Li XH
FAU - Liu, Zhi-Heng
AU  - Liu ZH
FAU - Liu, Gui-Jie
AU  - Liu GJ
FAU - Gao, Yan-Chao
AU  - Gao YC
FAU - Zhang, Xue-Li
AU  - Zhang XL
FAU - Sun, Hiu-Dong
AU  - Sun HD
LA  - eng
PT  - Journal Article
DEP - 20130904
PL  - England
TA  - Cell Mol Biol Lett
JT  - Cellular & molecular biology letters
JID - 9607427
RN  - 0 (HGF protein, human)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Engineering/methods
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Hepatocyte Growth Factor/*genetics/metabolism
MH  - Hepatocytes/cytology/*metabolism/*transplantation
MH  - Humans
MH  - Liver/cytology/*injuries/*physiology/surgery
MH  - *Liver Regeneration
MH  - Male
MH  - Plasmids/administration & dosage/genetics
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Transfection
PMC - PMC6275751
EDAT- 2013/09/06 06:00
MHDA- 2014/09/10 06:00
PMCR- 2013/09/04
CRDT- 2013/09/06 06:00
PHST- 2012/11/06 00:00 [received]
PHST- 2013/08/29 00:00 [accepted]
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
PHST- 2013/09/04 00:00 [pmc-release]
AID - 104 [pii]
AID - 10.2478/s11658-013-0104-1 [doi]
PST - ppublish
SO  - Cell Mol Biol Lett. 2013 Dec;18(4):507-21. doi: 10.2478/s11658-013-0104-1. Epub 
      2013 Sep 4.