PMID- 24005604
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20211021
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 405
IP  - 27
DP  - 2013 Nov
TI  - An analytical investigation of 24 oxygenated-PAHs (OPAHs) using liquid and gas 
      chromatography-mass spectrometry.
PG  - 8885-96
LID - 10.1007/s00216-013-7319-x [doi]
AB  - We developed two independent approaches for separation and quantitation of 24 
      oxygenated polycyclic aromatic hydrocarbons (OPAHs) using both liquid 
      chromatography-atmospheric pressure chemical ionization/mass spectrometry 
      (LC-APCI/MS) and gas chromatography-electron impact/mass spectrometry (GC-EI/MS). 
      Building on previous OPAH research, we examined laboratory stability of OPAHs, 
      improved existing method parameters, and compared quantification strategies using 
      standard addition and an internal standard on an environmental sample. Of 24 
      OPAHs targeted in this research, 19 compounds are shared between methods, with 3 
      uniquely quantitated by GC-EI/MS and 2 by LC-APCI/MS. Using calibration 
      standards, all GC-EI/MS OPAHs were within 15 % of the true value and had less 
      than 15 % relative standard deviations (RSDs) for interday variability. 
      Similarly, all LC-APCI/MS OPAHs were within 20 % of the true value and had less 
      than 15 % RSDs for interday variability. Instrument limits of detection ranged 
      from 0.18 to 36 ng mL(-1) on the GC-EI/MS and 2.6 to 26 ng mL(-1) on the 
      LC-APCI/MS. Four standard reference materials were analyzed with each method, and 
      we report some compounds not previously published in these materials, such as 
      perinaphthenone and xanthone. Finally, an environmental passive sampling extract 
      from Portland Harbor Superfund, OR was analyzed by each method using both 
      internal standard and standard addition to compensate for potential matrix 
      effects. Internal standard quantitation resulted in increased precision with 
      similar accuracy to standard addition for most OPAHs using 
      2-fluoro-fluorenone-(13)C as an internal standard. Overall, this work improves 
      upon OPAH analytical methods and provides some considerations and strategies for 
      OPAHs as focus continues to expand on this emerging chemical class.
FAU - O'Connell, Steven G
AU  - O'Connell SG
AD  - Environmental and Molecular Toxicology Department, Agricultural and Life Sciences 
      Building, Oregon State University, Rm 1007, Corvallis, OR, 97331, USA.
FAU - Haigh, Theodore
AU  - Haigh T
FAU - Wilson, Glenn
AU  - Wilson G
FAU - Anderson, Kim A
AU  - Anderson KA
LA  - eng
GR  - R21 ES020120/ES/NIEHS NIH HHS/United States
GR  - T32ES007060-32/ES/NIEHS NIH HHS/United States
GR  - P42 ES016465/ES/NIEHS NIH HHS/United States
GR  - T32 ES007060/ES/NIEHS NIH HHS/United States
GR  - P30 ES000210/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Validation Study
DEP - 20130905
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Solutions)
SB  - IM
MH  - Atmospheric Pressure
MH  - Calibration
MH  - Chromatography, Liquid/*standards
MH  - Environmental Pollutants/*isolation & purification
MH  - Gas Chromatography-Mass Spectrometry/*standards
MH  - Observer Variation
MH  - Polycyclic Aromatic Hydrocarbons/*isolation & purification
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solutions
PMC - PMC3824841
EDAT- 2013/09/06 06:00
MHDA- 2014/06/04 06:00
PMCR- 2013/09/05
CRDT- 2013/09/06 06:00
PHST- 2013/04/24 00:00 [received]
PHST- 2013/08/20 00:00 [accepted]
PHST- 2013/07/29 00:00 [revised]
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
PHST- 2013/09/05 00:00 [pmc-release]
AID - 7319 [pii]
AID - 10.1007/s00216-013-7319-x [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2013 Nov;405(27):8885-96. doi: 10.1007/s00216-013-7319-x. Epub 
      2013 Sep 5.