PMID- 24005606
OWN - NLM
STAT- MEDLINE
DCOM- 20140708
LR  - 20211021
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 176
IP  - 5-6
DP  - 2013 Dec
TI  - Paracoccidioides brasiliensis and P. lutzii antigens elicit different serum IgG 
      responses in chronic paracoccidioidomycosis.
PG  - 345-52
LID - 10.1007/s11046-013-9698-0 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the fungus 
      Paracoccidioides brasiliensis (S1, PS2, and PS3) and by the new species, P. 
      lutzii. Considering that genetic differences in the Paracoccidioides genus could 
      elicit distinct immune responses by the host, current research investigated serum 
      IgG levels to antigens from P. brasiliensis B339 (S1), P. brasiliensis LDR3 
      (PS2), and atypical strain LDR2 (P. lutzii), in patients with chronic PCM from 
      the northern and west regions of Parana, Brazil (n = 35). Cell-free antigen (CFA) 
      and high molecular mass fraction (hMM) were produced from each strain. Samples 
      were analyzed by ELISA and immunodiffusion (ID). ELISA positivity using CFA: 
      B339-100 %, LDR3-83 %, and LDR2-74 %. Response to CFA from B339 was more intense 
      (p < 0.05), while there was no difference between LDR3-LDR2. IgG anti-hMM was 
      higher for antigens from B339 or LDR3, when compared with LDR2 (p < 0.05). There 
      was a positive correlation for each strain between CFA-hMM and for hMM between 
      B339-LDR3 and LDR3-LDR2. ID positivity with CFA: B339-63 %, LDR3-66 %, and 
      LDR2-60 %. We conclude that the intensity of reaction of the patients' sera 
      varies with the strain used; hMM influences tests that use CFA, independently of 
      strain; using ID, positive rates were very similar, but there was a large number 
      of false negative results; ELISA tests using antigens from P. brasiliensis S1 
      were able to detect a larger number of patients than PS2 and P. lutzii (which had 
      a considerable number of false negative results), and therefore, its use may be 
      more appropriate in this region of Brazil.
FAU - Lenhard-Vidal, A
AU  - Lenhard-Vidal A
AD  - Department of Pathological Sciences, State University of Londrina, UEL, Londrina, 
      PR, Brazil.
FAU - Assolini, J P
AU  - Assolini JP
FAU - Ono, M A
AU  - Ono MA
FAU - Bredt, C S O
AU  - Bredt CS
FAU - Sano, A
AU  - Sano A
FAU - Itano, E N
AU  - Itano EN
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130905
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Antibodies, Fungal/*blood
MH  - *Antigens, Fungal/immunology
MH  - Brazil
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin G/*blood
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/diagnosis/*immunology/*microbiology
MH  - Sensitivity and Specificity
MH  - Serum/immunology
EDAT- 2013/09/06 06:00
MHDA- 2014/07/09 06:00
CRDT- 2013/09/06 06:00
PHST- 2013/04/18 00:00 [received]
PHST- 2013/08/17 00:00 [accepted]
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/07/09 06:00 [medline]
AID - 10.1007/s11046-013-9698-0 [doi]
PST - ppublish
SO  - Mycopathologia. 2013 Dec;176(5-6):345-52. doi: 10.1007/s11046-013-9698-0. Epub 
      2013 Sep 5.