PMID- 24005865
OWN - NLM
STAT- MEDLINE
DCOM- 20140319
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 9
DP  - 2013 Sep 3
TI  - Progressive changes in inflammatory and matrix adherence of bronchial epithelial 
      cells with persistent respiratory syncytial virus (RSV) infection (progressive 
      changes in RSV infection).
PG  - 18024-40
LID - 10.3390/ijms140918024 [doi]
AB  - In addition to the acute manifestations of respiratory syncytial virus (RSV), 
      persistent infection may be associated with long-term complications in the 
      development of chronic respiratory diseases. To understand the mechanisms 
      underlying RSV-induced long-term consequences, we established an in vitro RSV 
      (strain A2) infection model using human bronchial epithelial (16HBE) cells that 
      persists over four generations and analyzed cell inflammation and matrix 
      adherence. Cells infected with RSV at multiplicity of infection (MOI) 0.0067 
      experienced cytolytic or abortive infections in the second generation (G2) or G3 
      but mostly survived up to G4. Cell morphology, leukocyte and matrix adherence of 
      the cells did not change in G1 or G2, but subsequently, leukocyte adherence and 
      cytokine/chemokine secretion, partially mediated by intercellular adhesion 
      molecule-1 (ICAM-1), increased drastically, and matrix adherence, partially 
      mediated by E-cadherin, decreased until the cells died. Tumor necrosis factor-alpha 
      (TNF-alpha) secretion was inhibited by ICAM-1 antibody in infected-16HBE cells, 
      suggesting that positive feedback between TNF-alpha secretion and ICAM-1 expression 
      may be significant in exacerbated inflammation. These data demonstrate the 
      susceptibility of 16HBE cells to RSV and their capacity to produce long-term 
      progressive RSV infection, which may contribute to inflammation mobilization and 
      epithelial shedding.
FAU - Liu, Xiaoai
AU  - Liu X
AD  - Department of Physiology, School of Basic Medical Science, Central South 
      University, Changsha 410078, China. alicelxa@gmail.com
FAU - Qin, Xiaoqun
AU  - Qin X
FAU - Xiang, Yang
AU  - Xiang Y
FAU - Liu, Huijun
AU  - Liu H
FAU - Gao, Ge
AU  - Gao G
FAU - Qin, Ling
AU  - Qin L
FAU - Liu, Chi
AU  - Liu C
FAU - Qu, Xiangping
AU  - Qu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130903
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Blotting, Western
MH  - Cell Adhesion/genetics/physiology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/virology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Respiratory Syncytial Virus Infections/*immunology/virology
MH  - Respiratory Syncytial Viruses/pathogenicity
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC3794767
EDAT- 2013/09/06 06:00
MHDA- 2014/03/22 06:00
PMCR- 2013/09/01
CRDT- 2013/09/06 06:00
PHST- 2013/07/07 00:00 [received]
PHST- 2013/08/16 00:00 [revised]
PHST- 2013/08/21 00:00 [accepted]
PHST- 2013/09/06 06:00 [entrez]
PHST- 2013/09/06 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - ijms140918024 [pii]
AID - ijms-14-18024 [pii]
AID - 10.3390/ijms140918024 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Sep 3;14(9):18024-40. doi: 10.3390/ijms140918024.