PMID- 24007374
OWN - NLM
STAT- MEDLINE
DCOM- 20141211
LR  - 20240321
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 6
DP  - 2013 Sep 4
TI  - Genotype diversity of Mycobacterium isolates from children in Jimma, Ethiopia.
PG  - 352
LID - 10.1186/1756-0500-6-352 [doi]
AB  - BACKGROUND: Paediatric tuberculosis (TB) is poorly addressed in Ethiopia and 
      information about its magnitude and the genotype distribution of the causative 
      Mycobacterium tuberculosis strains responsible for its spread are scanty. 
      METHODS: Gastric lavage or sputum samples were collected from consecutively 
      enrolled TB suspect children visiting Jimma University Hospital in 2011 and 
      cultured on Middlebrook 7H11 and Lowenstein-Jensen media. Acid fast bacterial 
      (AFB) isolates were subjected to molecular typing targeting regions of difference 
      (RDs), 16S rDNA gene and the direct repeat (DR) region using multiplex polymerase 
      chain reaction (mPCR), gene sequencing and spoligotyping, respectively. Molecular 
      drug susceptibility testing of M. tuberculosis isolates was performed by 
      Genotype(R)MTBDRplus line probe assay (LPA) (Hain Life Sciences, Germany). RESULTS: 
      Gastric lavage (n = 43) or sputum (n = 58) samples were collected from 101 
      children and 31.7% (32/101) of the samples were positive for AFB by microscopy, 
      culture and/or PCR. Out of 25 AFB isolates, 60% (15/25) were identified as M. 
      tuberculosis by PCR, and 40% isolates (10/25) were confirmed to be 
      non-tuberculous mycobacteria (NTM) by genus typing and 16S rDNA gene sequencing. 
      Lineage classification assigned the M. tuberculosis strains into Euro-American 
      (EUA, 66.7%; 10/15), East-African-Indian (EAI; 2/15), East-Asian (EA; 1/15) and 
      Indio-Oceanic (IO; 1/15) lineages. Seven M. tuberculosis strains were new to the 
      SpolDB4 database. All of the M. tuberculosis isolates were susceptible to 
      isoniazid (INH) and rifampicin (RIF), except for one strain (of spoligotype 
      SIT-149 or T3_ETH family) which had a mutation at the inhA locus which often 
      confers resistance to INH (low level) and ethionamide. CONCLUSIONS: Analysis of 
      the genetic population structure of paediatric M. tuberculosis strains suggested 
      similarity with that of adults, indicating an on-going and active transmission of 
      M. tuberculosis from adults to children in Ethiopia. There were no 
      multidrug-resistant TB (MDR-TB) strains among the isolates.
FAU - Workalemahu, Bereket
AU  - Workalemahu B
AD  - Armauer Hansen Research Institute (AHRI/ALERT), Addis Ababa, Ethiopia. 
      bekicho@gmail.com.
FAU - Berg, Stefan
AU  - Berg S
FAU - Tsegaye, Wondewosen
AU  - Tsegaye W
FAU - Abdissa, Alemseged
AU  - Abdissa A
FAU - Girma, Tsinuel
AU  - Girma T
FAU - Abebe, Markos
AU  - Abebe M
FAU - Aseffa, Abraham
AU  - Aseffa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130904
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Antitubercular Agents)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - V83O1VOZ8L (Isoniazid)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antitubercular Agents/therapeutic use
MH  - Bacterial Typing Techniques
MH  - Child
MH  - Child, Preschool
MH  - Drug Resistance, Bacterial
MH  - Ethiopia/epidemiology
MH  - Gastric Lavage
MH  - *Genetic Variation
MH  - Genotype
MH  - Humans
MH  - Infant
MH  - Isoniazid/therapeutic use
MH  - Mycobacterium tuberculosis/classification/*genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Rifampin/therapeutic use
MH  - Sputum/microbiology
MH  - Tuberculosis, Pulmonary/drug therapy/*epidemiology/microbiology/transmission
PMC - PMC3766673
EDAT- 2013/09/07 06:00
MHDA- 2014/12/17 06:00
PMCR- 2013/09/04
CRDT- 2013/09/07 06:00
PHST- 2013/02/15 00:00 [received]
PHST- 2013/08/29 00:00 [accepted]
PHST- 2013/09/07 06:00 [entrez]
PHST- 2013/09/07 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
PHST- 2013/09/04 00:00 [pmc-release]
AID - 1756-0500-6-352 [pii]
AID - 10.1186/1756-0500-6-352 [doi]
PST - epublish
SO  - BMC Res Notes. 2013 Sep 4;6:352. doi: 10.1186/1756-0500-6-352.