PMID- 24007551
OWN - NLM
STAT- MEDLINE
DCOM- 20140729
LR  - 20211021
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 44
IP  - 1
DP  - 2013 Sep 4
TI  - Replication characteristics of porcine reproductive and respiratory syndrome 
      virus (PRRSV) European subtype 1 (Lelystad) and subtype 3 (Lena) strains in nasal 
      mucosa and cells of the monocytic lineage: indications for the use of new 
      receptors of PRRSV (Lena).
PG  - 73
LID - 10.1186/1297-9716-44-73 [doi]
AB  - Recently, it has been demonstrated that subtype 3 strains of European type 
      porcine reproductive and respiratory syndrome virus (PRRSV) are more 
      virulent/pathogenic than subtype 1 strains. This points to differences in the 
      pathogenesis. In the present study, a new polarized nasal mucosa explant system 
      was used to study the invasion of the low virulent subtype 1 PRRSV strain 
      Lelystad (LV) and the highly virulent subtype 3 PRRSV strain Lena at the portal 
      of entry. Different cell types of the monocytic lineage (alveolar macrophages 
      (PAM), cultured blood monocytes and monocyte-derived dendritic cells (moDC)) were 
      enclosed to examine replication kinetics of both strains in their putative target 
      cells. At 0, 12, 24, 48 and 72 hours post inoculation (hpi), virus production was 
      analyzed and the infected cells were quantified and identified. Lena replicated 
      much more efficiently than LV in the nasal mucosa explants and to a lesser extent 
      in PAM. Differences in replication were not found in monocytes and moDC. Confocal 
      microscopy demonstrated that for LV, almost all viral antigen positive cells were 
      CD163(+)Sialoadhesin (Sn)(+), which were mainly located in the lamina propria of the 
      respiratory mucosa. In Lena-infected nasal mucosa, CD163(+)Sn(+), CD163(+)Sn(-) and to a 
      lesser extent CD163(-)Sn(-) monocytic subtypes were involved in infection. CD163(+)Sn(-) 
      cells were mostly located within or in the proximity of the epithelium. Our 
      results show that, whereas LV replicates in a restricted subpopulation of 
      CD163(+)Sn(+) monocytic cells in the upper respiratory tract, Lena hijacks a broader 
      range of subpopulations to spread within the mucosa. Replication in CD163(+)Sn(-) 
      cells suggests that an alternative entry receptor may contribute to the wider 
      tropism of Lena.
FAU - Frydas, Ilias S
AU  - Frydas IS
AD  - Laboratory of Virology, Department of Virology, Immunology and Parasitology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke 
      B-9820, Belgium. hans.nauwynck@ugent.be.
FAU - Verbeeck, Mieke
AU  - Verbeeck M
FAU - Cao, Jun
AU  - Cao J
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130904
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/immunology
MH  - In Situ Nick-End Labeling/veterinary
MH  - Microscopy, Fluorescence/veterinary
MH  - Monocytes/immunology
MH  - Nasal Mucosa
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - *Virus Replication
PMC - PMC3849772
EDAT- 2013/09/07 06:00
MHDA- 2014/07/30 06:00
PMCR- 2013/01/01
CRDT- 2013/09/07 06:00
PHST- 2013/03/25 00:00 [received]
PHST- 2013/08/27 00:00 [accepted]
PHST- 2013/09/07 06:00 [entrez]
PHST- 2013/09/07 06:00 [pubmed]
PHST- 2014/07/30 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 1297-9716-44-73 [pii]
AID - 10.1186/1297-9716-44-73 [doi]
PST - epublish
SO  - Vet Res. 2013 Sep 4;44(1):73. doi: 10.1186/1297-9716-44-73.