PMID- 24008042
OWN - NLM
STAT- MEDLINE
DCOM- 20140325
LR  - 20231110
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 34
IP  - 36
DP  - 2013 Dec
TI  - The effect of cyclic mechanical strain on activation of dendritic cells cultured 
      on adhesive substrates.
PG  - 9063-70
LID - S0142-9612(13)00969-1 [pii]
LID - 10.1016/j.biomaterials.2013.08.021 [doi]
AB  - Dendritic cells (DCs), key regulators of tolerance and immunity, have been found 
      to reside in mechanically active tissues such as the interior layers of the 
      arterial wall, which experience cyclic radial wall strain due to pulsatile blood 
      flow. Although experimentally difficult to determine in vivo, it is reasonable to 
      postulate DCs experience the mechanical forces in such mechanically active 
      tissues. However, it is currently unknown how DCs respond to cyclic mechanical 
      strain. In order to explore the hypothesis that DCs are responsive to mechanical 
      strain, DCs were cultured in vitro on pre-adsorbed adhesive proteins (e.g., 
      laminin, collagen, fibrinogen) and 1 Hz cyclic strain was applied for various 
      durations and strain magnitudes. It was determined that a strain magnitude of 10% 
      and 24 h duration adversely affected DC viability compared to no-strain controls, 
      but culture on certain adhesive substrates provided modest protection of 
      viability under this harsh strain regime. In contrast, application of 1 h of 1 Hz 
      cyclic 3% strain did not affect DC viability and this strain regime was used for 
      the remaining experiments for quantifying DC activation and T-cell priming 
      capability. Application of 3% strain increased expression of stimulatory (MHC-II) 
      and costimulatory molecules (CD86, CD40), and this effect was generally increased 
      by culture on pre-coated adhesive substrates. Interestingly, the cytokine 
      secretion profile of DCs was not significantly affected by strain. Lastly, 
      strained DCs demonstrated increased stimulation of allogeneic T-cell 
      proliferation, in a manner that was independent of the adhesive substrate. These 
      observations indicate generation of a DC consistent with what has been described 
      as a semi-mature phenotype. This work begins elucidating a potential role for DCs 
      in tissue environments exposed to cyclic mechanical forces.
CI  - Published by Elsevier Ltd.
FAU - Lewis, Jamal S
AU  - Lewis JS
AD  - J. Crayton Pruitt Family Department of Biomedical Engineering, University of 
      Florida, Gainesville, FL 32611, USA.
FAU - Dolgova, Natalia V
AU  - Dolgova NV
FAU - Chancellor, Thomas J
AU  - Chancellor TJ
FAU - Acharya, Abhinav P
AU  - Acharya AP
FAU - Karpiak, Jerome V
AU  - Karpiak JV
FAU - Lele, Tanmay P
AU  - Lele TP
FAU - Keselowsky, Benjamin G
AU  - Keselowsky BG
LA  - eng
GR  - R01EB014869/EB/NIBIB NIH HHS/United States
GR  - R01 EB014869/EB/NIBIB NIH HHS/United States
GR  - R56DK091658/DK/NIDDK NIH HHS/United States
GR  - R01 DK091658/DK/NIDDK NIH HHS/United States
GR  - R56 DK091658/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130903
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Adhesives)
RN  - 0 (Cytokines)
RN  - 0 (Fibronectins)
RN  - 0 (Vitronectin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Adhesives/*pharmacology
MH  - Animals
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/drug effects/metabolism
MH  - Female
MH  - Fibronectins/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Serum Albumin, Bovine/pharmacology
MH  - *Stress, Mechanical
MH  - T-Lymphocytes/cytology/drug effects
MH  - Vitronectin/pharmacology
PMC - PMC4120880
MID - NIHMS604060
OTO - NOTNLM
OT  - Cell adhesion
OT  - Dendritic cells
OT  - Extracellular matrix
OT  - Immunology
OT  - Mechanical force
OT  - Mechanical strain
EDAT- 2013/09/07 06:00
MHDA- 2014/03/26 06:00
PMCR- 2014/08/04
CRDT- 2013/09/07 06:00
PHST- 2013/07/11 00:00 [received]
PHST- 2013/08/09 00:00 [accepted]
PHST- 2013/09/07 06:00 [entrez]
PHST- 2013/09/07 06:00 [pubmed]
PHST- 2014/03/26 06:00 [medline]
PHST- 2014/08/04 00:00 [pmc-release]
AID - S0142-9612(13)00969-1 [pii]
AID - 10.1016/j.biomaterials.2013.08.021 [doi]
PST - ppublish
SO  - Biomaterials. 2013 Dec;34(36):9063-70. doi: 10.1016/j.biomaterials.2013.08.021. 
      Epub 2013 Sep 3.