PMID- 24010007 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20130906 LR - 20211021 IS - 2156-7085 (Print) IS - 2156-7085 (Electronic) IS - 2156-7085 (Linking) VI - 4 IP - 8 DP - 2013 TI - Continuous real-time photoacoustic demodulation via field programmable gate array for dynamic imaging of zebrafish cardiac cycle. PG - 1451-63 LID - 10.1364/BOE.4.001451 [doi] AB - A four dimensional data set of the cardiac cycle of a zebrafish embryo was acquired using postacquisition synchronization of real time photoacoustic b-scans. Utilizing an off-axis photoacoustic microscopy (OA-PAM) setup, we have expanded upon our previous work with OA-PAM to develop a system that can sustain 100 kHz line rates while demodulating the bipolar photoacoustic signal in real-time. Real-time processing was accomplished by quadrature demodulation on a Field Programmable Gate Array (FPGA) in line with the signal digitizer. Simulated data acquisition verified the system is capable of real-time processing up to a line rate of 1 MHz. Galvanometer-scanning of the excitation laser inside the focus of the ultrasonic transducer enables real data acquisition of a 200 by 200 by 200 pixel, volumetric data set across a 2 millimeter field of view at a rate of 2.5 Hz. FAU - Mattison, Scott P AU - Mattison SP AD - Department of Biomedical Engineering, 5045 Emerging Technologies Building, 3120 TAMU, Texas A&M University, College Station, TX 7784, USA. FAU - Shelton, Ryan L AU - Shelton RL FAU - Maxson, Ryan T AU - Maxson RT FAU - Applegate, Brian E AU - Applegate BE LA - eng PT - Journal Article DEP - 20130729 PL - United States TA - Biomed Opt Express JT - Biomedical optics express JID - 101540630 PMC - PMC3756580 OTO - NOTNLM OT - (100.2000) Digital image processing OT - (110.5120) Photoacoustic imaging OT - (110.5125) Photoacoustics EDAT- 2013/09/07 06:00 MHDA- 2013/09/07 06:01 PMCR- 2013/07/29 CRDT- 2013/09/07 06:00 PHST- 2013/06/10 00:00 [received] PHST- 2013/07/22 00:00 [revised] PHST- 2013/07/23 00:00 [accepted] PHST- 2013/09/07 06:00 [entrez] PHST- 2013/09/07 06:00 [pubmed] PHST- 2013/09/07 06:01 [medline] PHST- 2013/07/29 00:00 [pmc-release] AID - 192020 [pii] AID - 10.1364/BOE.4.001451 [doi] PST - epublish SO - Biomed Opt Express. 2013 Jul 29;4(8):1451-63. doi: 10.1364/BOE.4.001451. eCollection 2013.