PMID- 24010021
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130906
LR  - 20220408
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 1
DP  - 2013
TI  - Hepatic bile acids and bile acid-related gene expression in pregnant and 
      lactating rats.
PG  - e143
LID - 10.7717/peerj.143 [doi]
LID - e143
AB  - Background. Significant physiological changes occur during pregnancy and 
      lactation. Intrahepatic cholestasis of pregnancy (ICP) is a liver disease closely 
      related to disruption of bile acid homeostasis. The objective of this study was 
      to examine the regulation of bile acid synthesis and transport in normal pregnant 
      and lactating rats. Materials and Methods. Livers from timed pregnant SD rats 
      were collected on gestational days (GD) 10, 14 and 19, and postnatal days (PND) 
      1, 7, 14 and 21. Total bile acids were determined by the enzymatic method, total 
      RNA was isolated and subjected to real time RT-PCR analysis. Liver protein was 
      extracted for western-blot analysis. Results. Under physiological conditions 
      hepatic bile acids were not elevated during pregnancy but increased during 
      lactation in rats. Bile acid synthesis rate-limiting enzyme Cyp7a1 was unchanged 
      on gestational days, but increased on PND14 and 21 at mRNA and protein levels. 
      Expression of Cyp8b1, Cyp27a1 and Cyp7b1 was also higher during lactation. The 
      mRNA levels of small heterodimer partner (SHP) and protein levels of farnesoid X 
      receptor (FXR) were increased during pregnancy and lactation. Bile acid 
      transporters Ntcp, Bsep, Mrp3 and Mrp4 were lower at gestation, but increased 
      during lactation. Hepatic Oatp transporters were decreased during pregnancy and 
      lactation. Conclusion. Hepatic bile acid homeostasis is maintained during normal 
      pregnancy in rats, probably through the FXR-SHP regulation. The expression of 
      bile acid synthesis genes and liver bile acid accumulation were increased during 
      lactation, together with increased expression of bile acid efflux transporter 
      Bsep, Mrp3 and Mrp4.
FAU - Zhu, Qiong N
AU  - Zhu QN
AD  - Department of Pharmacology and Key Lab of Basic Pharmacology of Guizhou, Zunyi 
      Medical College , Zunyi , China.
FAU - Xie, Hong M
AU  - Xie HM
FAU - Zhang, Dan
AU  - Zhang D
FAU - Liu, Jie
AU  - Liu J
FAU - Lu, Yuan F
AU  - Lu YF
LA  - eng
PT  - Journal Article
DEP - 20130827
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC3757468
OTO - NOTNLM
OT  - Cyp7a1
OT  - FXR-SHP
OT  - Liver bile acids
OT  - Ntcp and Bsep
OT  - Oatps
OT  - Pregnant and lactating rats
EDAT- 2013/09/07 06:00
MHDA- 2013/09/07 06:01
PMCR- 2013/08/27
CRDT- 2013/09/07 06:00
PHST- 2013/06/24 00:00 [received]
PHST- 2013/08/05 00:00 [accepted]
PHST- 2013/09/07 06:00 [entrez]
PHST- 2013/09/07 06:00 [pubmed]
PHST- 2013/09/07 06:01 [medline]
PHST- 2013/08/27 00:00 [pmc-release]
AID - 143 [pii]
AID - 10.7717/peerj.143 [doi]
PST - epublish
SO  - PeerJ. 2013 Aug 27;1:e143. doi: 10.7717/peerj.143. eCollection 2013.