PMID- 24010817
OWN - NLM
STAT- MEDLINE
DCOM- 20140807
LR  - 20231213
IS  - 1472-6882 (Electronic)
IS  - 1472-6882 (Linking)
VI  - 13
DP  - 2013 Sep 8
TI  - Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse 
      model through neurotrophin regulation.
PG  - 220
LID - 10.1186/1472-6882-13-220 [doi]
AB  - BACKGROUND: This study investigates the effect of Xiao-Qing-Long-Tang (XQLT) on 
      neurotrophin in an established mouse model of Dermatophagoides pteronyssinus (Der 
      p)-induced acute allergic asthma and in a LA4 cell line model of lung adenoma. 
      The effects of XQLT on the regulation of nerve growth factor (NGF) and 
      brain-derived neurotrophic factor (BDNF), airway hyper-responsiveness (AHR) and 
      immunoglobulin E were measured. METHODS: LA4 cells were stimulated with 100 mug/ml 
      Der p 24 h and the supernatant was collected for ELISA analysis. Der p-stimulated 
      LA4 cells with either XQLT pre-treatment or XQLT co-treatment were used to 
      evaluate the XQLT effect on neurotrophin.Balb/c mice were sensitized on days 0 
      and 7 with a base-tail injection of 50 mug Dermatophagoides pteronyssinus (Der p) 
      that was emulsified in 50 mul incomplete Freund's adjuvant (IFA). On day 14, mice 
      received an intra-tracheal challenge of 50 mul Der p (2 mg/ml). XQLT (1g/Kg) was 
      administered orally to mice either on days 2, 4, 6, 8, 10 and 12 as a preventive 
      strategy or on day 15 as a therapeutic strategy. RESULTS: XQLT inhibited 
      expression of those NGF, BDNF and thymus-and activation-regulated cytokine (TARC) 
      in LA4 cells that were subjected to a Der p allergen. Both preventive and 
      therapeutic treatments with XQLT in mice reduced AHR. Preventive treatment with 
      XQLT markedly decreased NGF in broncho-alveolar lavage fluids (BALF) and BDNF in 
      serum, whereas therapeutic treatment reduced only serum BDNF level. The reduced 
      NGF levels corresponded to a decrease in AHR by XQLT treatment. Reduced BALF NGF 
      and TARC and serum BDNF levels may have been responsible for decreased eosinophil 
      infiltration into lung tissue. Immunohistochemistry showed that p75NTR and TrkA 
      levels were reduced in the lungs of mice under both XQLT treatment protocols, and 
      this reduction may have been correlated with the prevention of the asthmatic 
      reaction by XQLT. CONCLUSION: XQLT alleviated allergic inflammation including 
      AHR, IgE elevation and eosinophil infiltration in Der p stimulated mice by 
      regulating neurotrophin and reducing TARC. These results revealed the potential 
      pharmacological targets on which the XQLT decotion exerts preventive and 
      therapeutic effects in an allergic asthma mouse model.
FAU - Chang, Ren-Shiu
AU  - Chang RS
AD  - School of Chinese Medicine, College of Chinese Medicine, China Medical 
      University, No, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. 
      stkao@mail.cmu.edu.tw.
FAU - Wang, Shulhn-Der
AU  - Wang SD
FAU - Wang, Yu-Chin
AU  - Wang YC
FAU - Lin, Li-Jen
AU  - Lin LJ
FAU - Kao, Shung-Te
AU  - Kao ST
FAU - Wang, Jiu-Yao
AU  - Wang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130908
PL  - England
TA  - BMC Complement Altern Med
JT  - BMC complementary and alternative medicine
JID - 101088661
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Protective Agents)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Ngfr protein, mouse)
RN  - 0 (sho-seiryu-to)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Airway Resistance/drug effects
MH  - Animals
MH  - Antigens, Dermatophagoides
MH  - Asthma/*metabolism
MH  - Brain-Derived Neurotrophic Factor/analysis/metabolism
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Cell Line, Tumor
MH  - Cytokines/analysis/metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Female
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Growth Factor/analysis/metabolism
MH  - Protective Agents/chemistry/*pharmacology
MH  - Receptors, Nerve Growth Factor/analysis/*metabolism
PMC - PMC3847146
EDAT- 2013/09/10 06:00
MHDA- 2014/08/08 06:00
PMCR- 2013/09/08
CRDT- 2013/09/10 06:00
PHST- 2013/03/07 00:00 [received]
PHST- 2013/09/06 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2014/08/08 06:00 [medline]
PHST- 2013/09/08 00:00 [pmc-release]
AID - 1472-6882-13-220 [pii]
AID - 10.1186/1472-6882-13-220 [doi]
PST - epublish
SO  - BMC Complement Altern Med. 2013 Sep 8;13:220. doi: 10.1186/1472-6882-13-220.