PMID- 24010821
OWN - NLM
STAT- MEDLINE
DCOM- 20151030
LR  - 20161125
IS  - 1748-1716 (Electronic)
IS  - 1748-1708 (Linking)
VI  - 210
IP  - 2
DP  - 2014 Feb
TI  - Activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and 
      Nrf-2-dependent genes by ischaemic pre-conditioning and post-conditioning: new 
      adaptive endogenous protective responses against renal ischaemia/reperfusion 
      injury.
PG  - 342-53
LID - 10.1111/apha.12164 [doi]
AB  - AIM: To investigate the impact of ischaemic pre-conditioning (Ipre) and 
      post-conditioning (Ipost) on expression of nuclear factor erythroid 2-related 
      factor 2 (Nrf2) gene and its dependent genes, haem oxygenase-1 (HO-1) and 
      NADPH-quinone oxidoreductase-1 (NQO-1); inflammatory cytokines TNF-alpha, IL1beta and 
      ICAM-1; and apoptotic markers such as caspase-3 in renal ischaemia/reperfusion 
      (I/R) injury. METHODS: One hundred and fifty male Sprague Dawley rats were 
      classified into five groups (each consisted of 30 rats): sham, control (I/R), 
      Ipre + I/R, Ipre without I/R and Ipost + I/R. Serum creatinine and blood urea 
      nitrogen (BUN) were measured at 2, 24 and 48 h after ischaemia. In kidney 
      tissues, mRNA of Nrf2, HO-1, NQO-1, TNF-alpha, IL-1beta and ICAM-1 and 
      immunohistochemical expression of Nrf2 and caspase-3 were assessed. RESULTS: 
      Serum creatinine and BUN improved significantly in Pre + I/R group; however, they 
      did not show any significant improvement in Post + I/R group. Also, Ipre-I/R 
      group showed non-significant change in serum creatinine and BUN. The expression 
      of Nrf2, HO-1 and NQO-1 is increased significantly in Pre + I/R and Pre - I/R 
      groups, while the enhancement in Post + I/R group was non-significant. Moreover, 
      the expression of proinflammatory cytokines (TNF-alpha, IL-1 and ICAM-1) and 
      apoptotic (caspase-3) markers showed high significant attenuation in Pre + I/R 
      group, but slight significant attenuation in Pre + I/R group. CONCLUSION: The 
      renoprotective action of Ipre might include early activation and enhanced 
      expression of Nrf2 gene and its dependent antioxidant genes, HO-1 and NOQ1, as 
      endogenous adaptive renoprotective genes, as well as reduction in TNF-alpha, IL-1beta, 
      ICAM-1 and caspase-3.
CI  - (c) 2013 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
FAU - Shokeir, A A
AU  - Shokeir AA
AD  - Urology and Nephrology Center, Faculty of Medicine, Mansoura University, 
      Mansoura, Egypt.
FAU - Hussein, A M
AU  - Hussein AM
FAU - Barakat, N
AU  - Barakat N
FAU - Abdelaziz, A
AU  - Abdelaziz A
FAU - Elgarba, M
AU  - Elgarba M
FAU - Awadalla, A
AU  - Awadalla A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131007
PL  - England
TA  - Acta Physiol (Oxf)
JT  - Acta physiologica (Oxford, England)
JID - 101262545
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, rat)
SB  - IM
MH  - Acute Kidney Injury/*metabolism
MH  - Adaptation, Physiological
MH  - Animals
MH  - Heme Oxygenase-1/metabolism
MH  - Immunohistochemistry
MH  - Ischemic Postconditioning/methods
MH  - Ischemic Preconditioning/*methods
MH  - Male
MH  - NAD(P)H Dehydrogenase (Quinone)/metabolism
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Reperfusion Injury/*metabolism
OTO - NOTNLM
OT  - HO-1
OT  - NQO-1
OT  - Nrf2
OT  - apoptosis
OT  - cytokines
OT  - ischaemia/reperfusion
OT  - post-conditioning
OT  - pre-conditioning
EDAT- 2013/09/10 06:00
MHDA- 2015/10/31 06:00
CRDT- 2013/09/10 06:00
PHST- 2013/02/07 00:00 [received]
PHST- 2013/02/25 00:00 [revised]
PHST- 2013/08/18 00:00 [revised]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2015/10/31 06:00 [medline]
AID - 10.1111/apha.12164 [doi]
PST - ppublish
SO  - Acta Physiol (Oxf). 2014 Feb;210(2):342-53. doi: 10.1111/apha.12164. Epub 2013 
      Oct 7.