PMID- 24011189
OWN - NLM
STAT- MEDLINE
DCOM- 20140324
LR  - 20211203
IS  - 2300-732X (Electronic)
IS  - 1642-431X (Linking)
VI  - 13
IP  - 3
DP  - 2013 Sep
TI  - The Akt/mTor signaling cascade is modified during placentation in the porcine 
      uterine tissue.
PG  - 184-94
LID - S1642-431X(13)00245-3 [pii]
LID - 10.1016/j.repbio.2013.06.001 [doi]
AB  - Recently we showed that essential components for the initiation of protein 
      synthesis, namely the eukaryotic initiation factor 4E (eIF4E, mRNA-cap-binding 
      protein) and its repressors 4E-BP1 as well as 4E-BP2, are proteolytically 
      processed in the porcine endometrium during implantation. Here, the situation 
      during placentation was compared with ovariectomized (OVX) animals and animals on 
      pregnancy day 1 (PD1). Furthermore, the research was extended to factors which 
      phosphorylate eIF4E and 4E-BPs and regulate their activities. These are the 
      protein kinase B/mammalian target of rapamycin kinase (Akt/mTor) with the 
      regulators Raptor and Rictor as well as the mitogen activated protein kinases 
      (MAPKs): extra cellular-signal regulated kinase 1 and 2 (ERK1 and ERK2). Striking 
      differences in the placentation site (PS) and the areas aside from PS (peri-PS) 
      were observed. EIF4E and 4E-BP2 truncation as well as 4E-BP1 degradation took 
      place in the endometrium of the peri-PS on PD24. Accompanied by a fragmentation 
      of Akt/mTor, no expression of Rictor was observed, whereas the abundance of 
      Raptor was not altered. On the contrary, MAPKs expression and phosphorylation 
      remained almost stable in the peri-PS. In conclusion, the results indicated that 
      on PD24 the translational regulation was shifted to 4E-BP2 control. Furthermore, 
      the Akt/mTor signaling cascade seemed to be down regulated which suggest reduced 
      phosphorylation of 4E-BP2. Whereas Akt was proteolyzed, the observed mTor 
      fragments represented most likely splicing variants. The results indicate that 
      translational control of gene expression is an important feature in the porcine 
      endometrium during early pregnancy.
CI  - Copyright (c) 2013 Society for Biology of Reproduction & the Institute of Animal 
      Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. 
      Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
FAU - Wollenhaupt, Karin
AU  - Wollenhaupt K
AD  - Leibniz Institute for Farm Animal Biology, Institute for Reproductive Biology, 
      D-18196 Dummerstorf, Germany.
FAU - Brussow, Klaus-Peter
AU  - Brussow KP
FAU - Albrecht, Dirk
AU  - Albrecht D
FAU - Tomek, Wolfgang
AU  - Tomek W
LA  - eng
PT  - Journal Article
DEP - 20130617
PL  - Poland
TA  - Reprod Biol
JT  - Reproductive biology
JID - 101160559
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Embryo Implantation/physiology
MH  - Eukaryotic Initiation Factor-4E/*physiology
MH  - Female
MH  - Phosphorylation
MH  - Placentation
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-akt/*physiology
MH  - Signal Transduction/*physiology
MH  - Swine
MH  - TOR Serine-Threonine Kinases/*physiology
MH  - Uterus/*metabolism
OTO - NOTNLM
OT  - Akt/mTor signaling
OT  - Placentation
OT  - Porcine uterine tissue
OT  - Translational control
OT  - eIF4E/4E-BPs
EDAT- 2013/09/10 06:00
MHDA- 2014/03/25 06:00
CRDT- 2013/09/10 06:00
PHST- 2013/02/07 00:00 [received]
PHST- 2013/06/03 00:00 [revised]
PHST- 2013/06/04 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2014/03/25 06:00 [medline]
AID - S1642-431X(13)00245-3 [pii]
AID - 10.1016/j.repbio.2013.06.001 [doi]
PST - ppublish
SO  - Reprod Biol. 2013 Sep;13(3):184-94. doi: 10.1016/j.repbio.2013.06.001. Epub 2013 
      Jun 17.