PMID- 24011916
OWN - NLM
STAT- MEDLINE
DCOM- 20140207
LR  - 20131209
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 186
IP  - 1
DP  - 2014 Jan
TI  - Therapeutic effects of p75 tumor necrosis factor receptor monoclonal antibody on 
      a rat model of traumatic arthritis.
PG  - 234-9
LID - S0022-4804(13)00720-8 [pii]
LID - 10.1016/j.jss.2013.07.047 [doi]
AB  - BACKGROUND: The aim of the present study was to investigate the therapeutic 
      effects of p75 tumor necrosis factor receptor monoclonal antibody D8F2 on a 
      traumatic arthritis model in rats, and to explore the underlying mechanism. 
      METHODS: Forty male Sprague Dawley rats were randomly divided into five groups: 
      (A) sham operation control group, (B) traumatic arthritis model group, (C) 
      low-dose D8F2 group (1 mg/kg), (D) medium-dose D8F2 group (3 mg/kg), and (E) 
      high-dose D8F2 group (10 mg/kg). Joint fluid samples were collected at 72 h after 
      surgery, and enzyme-linked immunosorbent assay was performed to measure the 
      following inflammatory factors: tumor necrosis factor alpha (TNF-alpha) and interleukin 
      1beta. One week after the surgery, rats were killed, and immunohistochemical 
      staining was applied to detect the matrix metalloproteinase (MMP1 and MMP3) 
      expression in the synovium. In cultured synovial fibroblast experiments, the 
      D8F2-induced ubiquitination of TNF receptor-associated factor 2 (TRAF2) was 
      examined by immunoprecipitation, and nuclear translocation of p65 nuclear 
      factor-kappaB (p65NF-kappaB) mediated by TNF-alpha and D8F2 was analyzed by western blotting. 
      RESULTS: In the traumatic arthritis model group, the inflammatory factors and 
      MMPs were significantly increased relative to the sham operation control group (P 
      < 0.05), whereas D8F2 could downregulate these factors in a dose-dependent manner 
      (P < 0.05). The results from in vitro studies indicated that D8F2 can induce 
      TRAF2 ubiquitination and inhibit the nuclear translocation of p65NF-kappaB mediated 
      by TNF-alpha. CONCLUSIONS: p75 Tumor necrosis factor receptor monoclonal antibody has 
      a therapeutic effect on traumatic arthritis, which may occur via the 
      downregulation of inflammatory factors and MMPs at the transcription level 
      because of TRAF2 degradation and inhibited activation of NF-kappaB.
CI  - Crown Copyright (c) 2014. Published by Elsevier Inc. All rights reserved.
FAU - Wang, Yi Xin
AU  - Wang YX
AD  - First-aid Center, Shanghai Putuo Hospital, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, PR China.
FAU - Xu, Kai
AU  - Xu K
FAU - Su, Wen Li
AU  - Su WL
FAU - You, Qing
AU  - You Q
FAU - Hu, Zhi Qian
AU  - Hu ZQ
FAU - Wang, Zhi Guo
AU  - Wang ZG
FAU - Zhu, Wen Xian
AU  - Zhu WX
FAU - Ruan, Can Ping
AU  - Ruan CP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130820
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*therapeutic use
MH  - Arthritis/*drug therapy
MH  - Cytokines/analysis
MH  - Disease Models, Animal
MH  - Joints/*injuries
MH  - Male
MH  - Matrix Metalloproteinase 1/analysis
MH  - NF-kappa B/antagonists & inhibitors
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Tumor Necrosis Factor, Type II/*antagonists & inhibitors/physiology
MH  - TNF Receptor-Associated Factor 2/metabolism
OTO - NOTNLM
OT  - Inflammatory factors
OT  - Matrix metalloproteinase
OT  - NF-kappaB
OT  - TRAF2
OT  - Traumatic arthritis
OT  - p75TNFR monoclonal antibody
EDAT- 2013/09/10 06:00
MHDA- 2014/02/08 06:00
CRDT- 2013/09/10 06:00
PHST- 2013/05/01 00:00 [received]
PHST- 2013/07/16 00:00 [revised]
PHST- 2013/07/24 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2014/02/08 06:00 [medline]
AID - S0022-4804(13)00720-8 [pii]
AID - 10.1016/j.jss.2013.07.047 [doi]
PST - ppublish
SO  - J Surg Res. 2014 Jan;186(1):234-9. doi: 10.1016/j.jss.2013.07.047. Epub 2013 Aug 
      20.