PMID- 24012967
OWN - NLM
STAT- MEDLINE
DCOM- 20140519
LR  - 20131011
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 150
IP  - 1
DP  - 2013 Oct 28
TI  - Eriosema laurentii De Wild (Leguminosae) methanol extract has estrogenic 
      properties and prevents menopausal symptoms in ovariectomized Wistar rats.
PG  - 298-307
LID - S0378-8741(13)00604-1 [pii]
LID - 10.1016/j.jep.2013.08.050 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Eriosema laurentii De Wild (Leguminosae) is a 
      medicinal plant used in West and Central Africa for different diseases. In 
      Cameroon, this plant is used as a treatment for infertility, and various 
      gynecological and menopausal complaints. However, despite this use as a natural 
      remedy, the biological activity of Eriosema laurentii has not been studied until 
      now. AIM OF STUDY: In order to determine the potential use of this plant in 
      gynecological conditions/disorders, we evaluated the estrogenic properties of a 
      methanol extract of its aerial parts and its ability to prevent different 
      menopausal health problems induced by bilateral oophorectomy. MATERIAL AND 
      METHODS: Two approaches were used. In vitro, recombinant yeast systems were 
      applied, featuring either the respective human receptors (ERalpha, AR, and PR) or 
      into chromosome III integrated human aryl hydrocarbon receptor (AhR) and the 
      respective reporter plasmid. In vivo, the investigation was carried out using the 
      3 days uterotrophic assay and 9 weeks oral treatment in ovariectomized rats. 
      RESULTS: The results showed that the methanol extract of the aerial parts of 
      Eriosema laurentii transactivated the estrogen receptor-alpha and displayed AhR 
      agonistic activity but was neither androgenic nor progesteronic. In rats, the 
      extract did not induce endometrium proliferation either in the 3-day or the 
      9-week treatment regimens, but induced vaginal stratification and cornification, 
      prevented loss of femur bone mass, increased high density lipoprotein cholesterol 
      (HDL-C), and reduced total cholesterol (TC), low density lipoprotein cholesterol 
      (LDL-C), TC/HDL-C ratio, LDL-C/HDL-C ratio and the atherogenic index of plasma 
      (AIP). CONCLUSION: These results suggest that the methanol extract of the aerial 
      parts of Eriosema laurentii does not seem to have an undesirable influence on the 
      endometrium but might prevent vaginal dryness and bone mass loss and improve the 
      lipid profile.
CI  - (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Ateba, Sylvin Benjamin
AU  - Ateba SB
AD  - Laboratory of Animal Physiology, Department of Animal Biology and Physiology, 
      Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon; 
      Department of Pharmacognosy, University of Vienna, Althanstrasse 14, A-1090 
      Vienna, Austria.
FAU - Njamen, Dieudonne
AU  - Njamen D
FAU - Medjakovic, Svjetlana
AU  - Medjakovic S
FAU - Hobiger, Stefanie
AU  - Hobiger S
FAU - Mbanya, Jean Claude
AU  - Mbanya JC
FAU - Jungbauer, Alois
AU  - Jungbauer A
FAU - Krenn, Liselotte
AU  - Krenn L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130905
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Flavonoids)
RN  - 0 (Phytoestrogens)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Solvents)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Epithelium/anatomy & histology/drug effects
MH  - Estrogen Receptor alpha/metabolism
MH  - *Fabaceae
MH  - Female
MH  - Femur/anatomy & histology/drug effects
MH  - Flavonoids/isolation & purification
MH  - Humans
MH  - Menopause/*drug effects/physiology
MH  - Methanol/chemistry
MH  - Organ Size/drug effects
MH  - Ovariectomy
MH  - Phytoestrogens/isolation & purification/*pharmacology
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Rats, Wistar
MH  - Receptors, Aryl Hydrocarbon/metabolism
MH  - Solvents/chemistry
MH  - Uterus/anatomy & histology/drug effects
MH  - Vagina/anatomy & histology/drug effects
MH  - beta-Galactosidase/metabolism
OTO - NOTNLM
OT  - 17beta-estradiol
OT  - AEL
OT  - AIP
OT  - AR
OT  - AhR
OT  - Aryl hydrocarbon receptor agonistic activity
OT  - Atherogenic index
OT  - CE
OT  - CHD
OT  - CVD
OT  - E2
OT  - E2B
OT  - E2V
OT  - ERalpha
OT  - Eriosema laurentii De Wild
OT  - Estrogenic activity
OT  - HDL-C
OT  - HPLC-DAD
OT  - LDL-C
OT  - ONPG
OT  - OVX
OT  - Osteoporosis
OT  - PR
OT  - Rt
OT  - Sham
OT  - TC
OT  - TG
OT  - UEH
OT  - UWW
OT  - VEH
OT  - VLDL
OT  - androgen receptor
OT  - aryl hydrocarbon receptor
OT  - atherogenic index of plasma
OT  - b-NF
OT  - bilaterally ovariectomized rats
OT  - cardiovascular disease
OT  - cholesteryl ester
OT  - coronary heart disease
OT  - estradiol benzoate
OT  - estradiol valerate
OT  - estrogen receptor alpha
OT  - high density lipoprotein cholesterol
OT  - high performance liquid chromatography-diode array detector
OT  - low density lipoprotein cholesterol
OT  - methanol extract of the aerial parts of Eriosema laurentii De Wild
OT  - o-nitrophenyl-beta-galactopyranoside
OT  - progesterone receptor
OT  - retention time
OT  - sham operated animals
OT  - total cholesterol
OT  - triglycerides
OT  - uterine epithelial height
OT  - uterine wet weight
OT  - vaginal epithelial height
OT  - very low density lipoprotein.
OT  - beta-naphthoflavone
EDAT- 2013/09/10 06:00
MHDA- 2014/05/20 06:00
CRDT- 2013/09/10 06:00
PHST- 2013/05/27 00:00 [received]
PHST- 2013/07/18 00:00 [revised]
PHST- 2013/08/23 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2014/05/20 06:00 [medline]
AID - S0378-8741(13)00604-1 [pii]
AID - 10.1016/j.jep.2013.08.050 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Oct 28;150(1):298-307. doi: 10.1016/j.jep.2013.08.050. 
      Epub 2013 Sep 5.