PMID- 24013624
OWN - NLM
STAT- MEDLINE
DCOM- 20151116
LR  - 20211021
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 30
IP  - 3
DP  - 2015 Apr
TI  - Effects of laser therapy on the proliferation of human periodontal ligament stem 
      cells.
PG  - 1171-4
LID - 10.1007/s10103-013-1436-9 [doi]
AB  - Low-level laser irradiation (LLLI) stimulates the proliferation of a variety of 
      cell types. However, very little is known about the effect of laser therapy on 
      dental stem cells. The aim of the present study was to evaluate the effect of 
      LLLI (660 nm, 30 mW) on the proliferation rate of human periodontal ligament stem 
      cells (hPDLSC), obtained from two healthy permanent third molars extracted due to 
      surgical indication. Culture cells were either irradiated or not (control) with 
      an InGaAIP diode laser at 0 and 48 h, using two different energy densities (0.5 
      J/cm(2), 16 s and 1.0 J/cm(2), 33 s). Cell proliferation was evaluated by the Trypan 
      blue exclusion method and by measuring mitochondrial activity using the MTT-based 
      cytotoxicity assay at intervals of 0, 24, 48, and 72 h after the first laser 
      application. An energy density of 1.0 J/cm(2) improved the cell proliferation in 
      comparison to the other groups (control and laser 0.5 J/cm(2)) at 48 and 72 h. The 
      group irradiated with 1.0 J/cm(2) presented significantly higher MTT activity at 48 
      and 72 h when compared to the energy density of 0.5 J/cm(2). It can be concluded 
      that LLLI using infrared light and an energy density of 1.0 J/cm(2) has a positive 
      stimulatory effect on the proliferation of hPDLSC.
FAU - Soares, Diego Moura
AU  - Soares DM
AD  - Federal University of Rio Grande do Norte, Natal, RN, Brazil.
FAU - Ginani, Fernanda
AU  - Ginani F
FAU - Henriques, Aguida Gomes
AU  - Henriques AG
FAU - Barboza, Carlos Augusto Galvao
AU  - Barboza CA
LA  - eng
PT  - Journal Article
DEP - 20130907
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
SB  - IM
MH  - Adult Stem Cells/*physiology/radiation effects
MH  - Cell Proliferation/*radiation effects
MH  - Cells, Cultured
MH  - Humans
MH  - *Lasers, Semiconductor
MH  - *Low-Level Light Therapy
MH  - Periodontal Ligament/*cytology
EDAT- 2013/09/10 06:00
MHDA- 2015/11/17 06:00
CRDT- 2013/09/10 06:00
PHST- 2013/07/01 00:00 [received]
PHST- 2013/08/28 00:00 [accepted]
PHST- 2013/09/10 06:00 [entrez]
PHST- 2013/09/10 06:00 [pubmed]
PHST- 2015/11/17 06:00 [medline]
AID - 10.1007/s10103-013-1436-9 [doi]
PST - ppublish
SO  - Lasers Med Sci. 2015 Apr;30(3):1171-4. doi: 10.1007/s10103-013-1436-9. Epub 2013 
      Sep 7.