PMID- 24018663
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20130925
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 77
IP  - 9
DP  - 2013
TI  - Purification and characterization of UDP-arabinopyranose mutase from 
      Chlamydomonas reinhardtii.
PG  - 1874-8
AB  - Chlamydomonas reinhardtii cells are surrounded by a mixture of hydroxyprolin-rich 
      glycoproteins consisting of L-arabinose, D-galactose, D-glucose, and D-mannose 
      residues. The L-arabinose residue is thought to be attached by a transfer of 
      UDP-L-arabinofuranose (UDP-Araf), which is produced from UDP-L-arabinopyranose 
      (UDP-Arap) by UDP-arabinopyranose mutase (UAM). UAM was purified from the cytosol 
      to determine the involvement of C. reinhardtii UAM (CrUAM) in glycoprotein 
      synthesis. CrUAM was purified 94-fold to electrophoretic homogeneity by 
      hydrophobic and size-exclusion chromatography. CrUAM catalyzed the reversible 
      conversion between UDP-Arap and UDP-Araf and exhibited autoglycosylation activity 
      when UDP-D-[(14)C]glucose was added as substrate. Compared to the properties of 
      native and recombinant CrUAM overexpressed in Escherichia coli, native CrUAM 
      showed a higher affinity for UDP-Arap than recombinant CrUAM did. This increased 
      affinity for UDP-Arap might have been caused by post-translational modifications 
      that occur in eukaryotes but not in prokaryotes.
FAU - Kotani, Ayana
AU  - Kotani A
AD  - Faculty of Agriculture, University of the Ryukyus.
FAU - Tsuji, Maki
AU  - Tsuji M
FAU - Azama, Yasushi
AU  - Azama Y
FAU - Ishii, Tadashi
AU  - Ishii T
FAU - Takeda, Takumi
AU  - Takeda T
FAU - Yamashita, Tetsuro
AU  - Yamashita T
FAU - Shimojima, Mie
AU  - Shimojima M
FAU - Konishi, Teruko
AU  - Konishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130907
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Recombinant Proteins)
RN  - 0 (Uridine Diphosphate Sugars)
RN  - 14697-41-7 (uridine diphosphate arabinose)
RN  - EC 5.4.- (Intramolecular Transferases)
SB  - IM
MH  - Chlamydomonas reinhardtii/cytology/*enzymology
MH  - Intramolecular Transferases/genetics/*isolation & purification/*metabolism
MH  - Kinetics
MH  - Recombinant Proteins/genetics/isolation & purification/metabolism
MH  - Substrate Specificity
MH  - Uridine Diphosphate Sugars/*metabolism
EDAT- 2013/09/11 06:00
MHDA- 2014/04/23 06:00
CRDT- 2013/09/11 06:00
PHST- 2013/09/11 06:00 [entrez]
PHST- 2013/09/11 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
AID - DN/JST.JSTAGE/bbb/130302 [pii]
AID - 10.1271/bbb.130302 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2013;77(9):1874-8. doi: 10.1271/bbb.130302. Epub 2013 
      Sep 7.