PMID- 24019628
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130910
LR  - 20220408
IS  - 0971-6866 (Print)
IS  - 1998-362X (Electronic)
IS  - 1998-362X (Linking)
VI  - 19
IP  - 2
DP  - 2013 Apr
TI  - Association between PRO12ALA polymorphism of the PPAR-gamma2 gene and type 2 diabetes 
      mellitus in Iranian patients.
PG  - 239-44
LID - 10.4103/0971-6866.116126 [doi]
AB  - BACKGROUND: Peroxisome proliferator-activated receptor (PPARs) have been 
      identified as ligand-activated transcription factors that belong to the nuclear 
      receptor superfamily. It has been shown that an association exists between 
      Proline 12 alanine (Pro12Ala) polymorphism of PPAR-GAMMA2 (PPAR-gamma2) gene and 
      increased risk of type 2 diabetes mellitus (T2DM) in different populations. 
      Therefore, the present study was designed to investigate the association between 
      Pro12Ala polymorphism of PPAR-gamma2 gene and T2DM in an Iranian population. 
      MATERIALS AND METHODS: Two hundred unrelated people, including 100 healthy 
      controls and 100 diabetic patients were recruited diagnosed based on American 
      Diabetes Association criteria. Blood samples were used for isolation of genomic 
      deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood 
      leukocytes by means of High Pure polymerase chain reaction (PCR) Template 
      preparation kit, we carried out polymerase chain reaction-restriction fragment 
      length polymorphism (PCR-RFLP) on each blood sample. Then, Genomic DNA was 
      digested by BstU-I restriction enzyme. Thereafter, restriction products were 
      analyzed by means of Polyacrylamide gel electrophoresis and stained by Ethidium 
      Bromide. RESULTS: We found that the frequency of Ala allele in healthy subjects 
      was significantly higher than in diabetic subjects (P = 0003). Moreover, the 
      genotype frequency of Ala/Ala in healthy subjects was significantly higher than 
      in diabetic subjects (P < 0.001). However, the genotype frequency of Ala/Pro in 
      diabetic subjects was significantly higher than in healthy subjects (P < 0.001). 
      CONCLUSION: The present study suggests that polymorphism of PPAR-gamma2 gene is 
      associated with T2DM. Furthermore, Ala allele is significantly found in 
      non-diabetic individual's Iranian population.
FAU - Motavallian, Azadeh
AU  - Motavallian A
AD  - Department of Pharmacology, School of Medicine, Guilan University of Medical 
      Sciences, Rasht, Iran.
FAU - Andalib, Sasan
AU  - Andalib S
FAU - Vaseghi, Golnaz
AU  - Vaseghi G
FAU - Mirmohammad-Sadeghi, Hamid
AU  - Mirmohammad-Sadeghi H
FAU - Amini, Masoud
AU  - Amini M
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Hum Genet
JT  - Indian journal of human genetics
JID - 101223637
PMC - PMC3758733
OTO - NOTNLM
OT  - Polymorphism
OT  - peroxisome proliferator-activated receptor-GAMMA 2 gene
OT  - type 2 diabetes mellitus
COIS- Conflict of Interest: None declared.
EDAT- 2013/09/11 06:00
MHDA- 2013/09/11 06:01
PMCR- 2013/04/01
CRDT- 2013/09/11 06:00
PHST- 2013/09/11 06:00 [entrez]
PHST- 2013/09/11 06:00 [pubmed]
PHST- 2013/09/11 06:01 [medline]
PHST- 2013/04/01 00:00 [pmc-release]
AID - IJHG-19-239 [pii]
AID - 10.4103/0971-6866.116126 [doi]
PST - ppublish
SO  - Indian J Hum Genet. 2013 Apr;19(2):239-44. doi: 10.4103/0971-6866.116126.