PMID- 24020178
OWN - NLM
STAT- MEDLINE
DCOM- 20131108
LR  - 20131121
IS  - 1026-3543 (Print)
IS  - 1026-3543 (Linking)
VI  - 143
IP  - 3
DP  - 2013
TI  - [Reactive changes of the rat brain cellular elements under different conditions 
      of circulatory hypoxia].
PG  - 14-21
AB  - The aim of this study was to detect structural, spatial and quantitative changes 
      of cellular elements of midbrain paranigral nucleus (PNN) and telencephalic 
      anterior cingulate area (ACA) under different conditions of circulatory hypoxia. 
      PNN anteriormedial part and ACA layers V-VI were examined in adult rats 7 days 
      (n=4) after an occlusion of both common carotid arteries as well as in intact 
      (1st control, n=4) and sham-operated animals (2nd control, n=4). In histological 
      the sections, stained with Nissl cresyl violet, and using the methods of glial 
      fibrillary acidic protein and an Ibal-protein detection, the proportions of 
      unmodified, hypochromic, pyknomorphic neurons and ghost cells were determined as 
      well as the numbers of astrocytes, oligodendrocytes, microgliocytes and 
      endotheliocytes. Cell body area of neurons and gliocytes, and the distance 
      between cell bodies and capillaries were measured, a gliocyte-neuronal index was 
      calculated. It was found that brain cellular elements that survive different 
      conditions of a circulatory hypoxia underwent a range of pathological changes. 
      Neurons were in process of nuclear pyknosis, lysis and transformation into the 
      ghost cells. The cells within the hypoxia nuclear zone were prone to death or 
      pyknosis. The neurons located outside the area of hypoxia which were affected 
      only by a humoral impact of reactions of the glutamate-calcium cascade, 
      frequently underwent acute swelling. Microgliocyte reaction in the form of poorly 
      expressed increase in their number and structural signs of activation was an 
      early diffuse manifestation of a prosencephalic focal hypoxia. Endotheliocyte 
      proliferation 7 days after of ischemic challenge was not associated with a chain 
      of cascade reactions and was observed only in the hypoxia focus. Concentration of 
      viable neurons and astrocytes near blood capillaries, as well as an increase in 
      the number of satellite form gliocytes is an adaptation mechanism and a condition 
      for the survival of cells during various types of brain exposure to ischemia.
FAU - Droblenkov, A V
AU  - Droblenkov AV
AD  - Department of Histology and Embryology, St. Petersburg State Pediatric Medical 
      University, St. Petersburg, Russia
FAU - Naumov, N V
AU  - Naumov NV
FAU - Monid, M V
AU  - Monid MV
FAU - Valkovich, E I
AU  - Valkovich EI
FAU - Shabanov, P D
AU  - Shabanov PD
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Morfologiia
JT  - Morfologiia (Saint Petersburg, Russia)
JID - 9317610
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Astrocytes/pathology
MH  - Calcium/metabolism
MH  - Capillaries/pathology
MH  - Carotid Artery Diseases/metabolism/*pathology
MH  - Cell Death
MH  - Endothelial Cells/pathology
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Glutamic Acid/metabolism
MH  - Hypoxia, Brain/*pathology
MH  - Male
MH  - Mesencephalon/blood supply/metabolism/*pathology
MH  - Microglia/pathology
MH  - Neurons/metabolism/*pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Telencephalon/blood supply/metabolism/*pathology
EDAT- 2013/09/12 06:00
MHDA- 2013/11/10 06:00
CRDT- 2013/09/12 06:00
PHST- 2013/09/12 06:00 [entrez]
PHST- 2013/09/12 06:00 [pubmed]
PHST- 2013/11/10 06:00 [medline]
PST - ppublish
SO  - Morfologiia. 2013;143(3):14-21.