PMID- 24022071
OWN - NLM
STAT- MEDLINE
DCOM- 20131219
LR  - 20131025
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 162
IP  - 3
DP  - 2013
TI  - Human leukocyte antigen-G and regulatory T cells during specific immunotherapy 
      for pollen allergy.
PG  - 237-52
LID - 10.1159/000353281 [doi]
AB  - BACKGROUND: TH2-biased immune responses are important in allergy pathogenesis. 
      Mechanisms of allergen-specific immunotherapy (SIT) might include the induction 
      of regulatory T cells (Tregs) and immunoglobulin (Ig) G4 blocking antibodies, a 
      reduction in the number of effector cells, and skewing of the cytokine profile 
      towards a TH1-polarized immune response. We investigated the effects of SIT on T 
      cells, on immunomodulation of human leukocyte antigen (HLA)-G, which has been 
      associated with allergy, on regulatory cytokine expression, and on serum 
      allergen-specific antibody subclasses (IgE and IgG4). METHODS: Eleven birch 
      and/or grass pollen-allergic patients and 10 healthy nonatopic controls were 
      studied before and during SIT. Tregs, chemokine receptors, soluble HLA-G 
      (sHLA-G), Ig-like transcript (ILT) 2, specific IgE, and IgG4 were studied. 
      Peripheral blood mononuclear cells (PBMCs) were stimulated with pollen extract in 
      vitro and immune factors were evaluated. RESULTS: During SIT, the main changes in 
      the peripheral blood were an increase in CXCR3(+)CD4(+)CD25(+)CD127(low/-) Tregs 
      and a decrease in CCR4(+)CD4(+)CD25(+)CD127(low/-) Tregs, an increase in 
      allergen-specific IgG4, and a decrease in sHLA-G during the first half of the 
      treatment period. In the PBMC in vitro experiments, the following changes were 
      observed upon allergen-stimulation: an increase in CD4(+)CD25(+)CD127(low/-) 
      Tregs and ILT2(+)CD4(+)CD25(+)CD127(low/-) Tregs, an increase in IL-10 and IL-2 
      levels, and an increase in sHLA-G that was most pronounced at the start of SIT. 
      CONCLUSIONS: The changes in CXCR3(+)CD4(+)CD25(+)CD127(low/-) Treg, IgG4, and 
      sHLA-G levels in the peripheral blood and in ILT2(+) Treg, IL-10, IL-2, and 
      sHLA-G levels upon in vitro allergen stimulation suggest an upregulation in 
      immunomodulatory factors and, to some degree, a shift towards TH1 during SIT.
CI  - Copyright (c) 2013 S. Karger AG, Basel.
FAU - Sorensen, Anja E
AU  - Sorensen AE
AD  - Department of Clinical Biochemistry, Centre for Immune Regulation and 
      Reproductive Immunology (CIRRI), Copenhagen University Hospital (Roskilde) and 
      Roskilde Hospital, Roskilde, Denmark.
FAU - Johnsen, Claus R
AU  - Johnsen CR
FAU - Dalgaard, Louise T
AU  - Dalgaard LT
FAU - Wurtzen, Peter Adler
AU  - Wurtzen PA
FAU - Kristensen, Bjarne
AU  - Kristensen B
FAU - Larsen, Margit Horup
AU  - Larsen MH
FAU - Ullum, Henrik
AU  - Ullum H
FAU - Soes-Petersen, Ulrik
AU  - Soes-Petersen U
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130906
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, CCR4)
RN  - 0 (Receptors, CXCR3)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Cytokines/biosynthesis
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Female
MH  - HLA-G Antigens/blood/*immunology
MH  - Humans
MH  - Immunoglobulin G/blood/immunology
MH  - Immunophenotyping
MH  - *Immunotherapy
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Pollen/*immunology
MH  - Receptors, CCR4/metabolism
MH  - Receptors, CXCR3/metabolism
MH  - Rhinitis, Allergic, Seasonal/*immunology/*therapy
MH  - T-Lymphocytes, Regulatory/*immunology/metabolism
MH  - Young Adult
EDAT- 2013/09/12 06:00
MHDA- 2013/12/20 06:00
CRDT- 2013/09/12 06:00
PHST- 2012/11/28 00:00 [received]
PHST- 2013/05/24 00:00 [accepted]
PHST- 2013/09/12 06:00 [entrez]
PHST- 2013/09/12 06:00 [pubmed]
PHST- 2013/12/20 06:00 [medline]
AID - 000353281 [pii]
AID - 10.1159/000353281 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2013;162(3):237-52. doi: 10.1159/000353281. Epub 2013 
      Sep 6.