PMID- 24022623
OWN - NLM
STAT- MEDLINE
DCOM- 20140203
LR  - 20211021
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 33
IP  - 12
DP  - 2013 Dec
TI  - Uncoupling PSD-95 interactions leads to rapid recovery of cortical function after 
      focal stroke.
PG  - 1937-43
LID - 10.1038/jcbfm.2013.153 [doi]
AB  - Since the most significant ischemic sequelae occur within hours of stroke, it is 
      necessary to understand how neuronal function changes during this time. While 
      histologic and behavioral models show the extent of stroke-related damage, only 
      in vivo recordings can illustrate changes in brain activity during stroke and 
      validate effectiveness of neuroprotective compounds. Spontaneous and evoked field 
      potentials (fEPs) were recorded in the deep layers of the cortex with a linear 
      microelectrode array for 3 hours after focal stroke in anesthetized rats. 
      Tat-NR2B9c peptide, which confers neuroprotection by uncoupling the PSD-95 
      protein from N-methyl-D-aspartate receptor (NMDAR), was administered 5 minutes 
      before ischemia. Evoked field potentials were completely suppressed within 
      3 minutes of infarct in all ischemic groups. Evoked field potential recovery 
      after stroke in rats treated with Tat-NR2B9c (83% of baseline) was greater 
      compared with stroke-only (61% of baseline) or control peptide (Tat-NR2B-AA; 67% 
      of baseline) groups (P<0.001). Electroencephalography (EEG) power was higher in 
      Tat-NR2B9c-treated animals at both 20 minutes and 1 hour (50% and 73% of 
      baseline, respectively) compared with stroke-only and Tat-NR2B-AA-treated rats 
      (P<0.05). Tat-NR2B9c significantly reduces stroke-related cortical dysfunction as 
      evidenced by greater recovery of fEPs and EEG power; illustrating the immediate 
      effects of the compound on poststroke brain function.
FAU - Srejic, Luka R
AU  - Srejic LR
AD  - Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada.
FAU - Hutchison, William D
AU  - Hutchison WD
FAU - Aarts, Michelle M
AU  - Aarts MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130911
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Peptides)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - D45TI2TWMA (Tat-NR2B9c)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*drug therapy/*physiopathology
MH  - Cerebral Cortex/*drug effects/*physiopathology
MH  - Disks Large Homolog 4 Protein
MH  - Evoked Potentials/drug effects
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Neuroprotective Agents/*therapeutic use
MH  - Peptides/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
PMC - PMC3851903
EDAT- 2013/09/12 06:00
MHDA- 2014/02/04 06:00
PMCR- 2014/12/01
CRDT- 2013/09/12 06:00
PHST- 2013/04/10 00:00 [received]
PHST- 2013/07/23 00:00 [revised]
PHST- 2013/07/24 00:00 [accepted]
PHST- 2013/09/12 06:00 [entrez]
PHST- 2013/09/12 06:00 [pubmed]
PHST- 2014/02/04 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - jcbfm2013153 [pii]
AID - 10.1038/jcbfm.2013.153 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2013 Dec;33(12):1937-43. doi: 10.1038/jcbfm.2013.153. 
      Epub 2013 Sep 11.