PMID- 24023309 OWN - NLM STAT- MEDLINE DCOM- 20131127 LR - 20210816 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 33 IP - 9 DP - 2013 Sep TI - ESR1 gene amplification in endometrial carcinomas: a clinicopathological analysis. PG - 3775-81 AB - This study investigated the clinicopathological significance of estrogen receptor 1 (ESR1) gene amplification and its relationship to phosphatase and tensin homolog (PTEN), human epidermal growth factor receptor 2 (HER2), MutL homolog 1 (MLH1), p53, and AT rich interactive domain 1A (ARID1A) expression in endometrial carcinomas. ESR1 amplification and expression were assessed by fluorescence in situ hybridization and immunohistochemistry. Clinical data were collected by retrospective chart review. ESR1 amplification was identified in 13 out of 111 (11.7%) endometrial carcinomas. No significant association was observed between ESR1 amplification and International Federation of Gynecology and Obstetrics (FIGO) stage (p=0.17), histological grade (p=0.35), lymph node metastasis (p=0.51), or deep myometrial invasion (p=0.46). ESR1 amplification was independent of PTEN, p53, HER2, MLH1, and ARID1A protein expression. Patients without estrogen receptor (ER) or progesterone receptor (PR) expression had shorter progression-free and overall survival than those with ER or PR expression (p<0.01). ESR1 amplification is independent of known clinicopathological factors related to poor prognosis and PTEN, p53, HER2, MLH1, and ARID1A protein expression, suggesting ESR1 amplification may be an early event in endometrial carcinoma development. FAU - Rahman, Mohammed Tanjimur AU - Rahman MT AD - Shimane University School of Medicine, Enyacho 89-1, Izumo, Shimane, 6938501 Japan. kn88@medshimane-u.ac.jp. FAU - Nakayama, Kentaro AU - Nakayama K FAU - Rahman, Munmun AU - Rahman M FAU - Ishikawa, Masako AU - Ishikawa M FAU - Katagiri, Hiroshi AU - Katagiri H FAU - Katagiri, Atsuko AU - Katagiri A FAU - Ishibashi, Tomoka AU - Ishibashi T FAU - Sato, Emi AU - Sato E FAU - Iida, Kouji AU - Iida K FAU - Ishikawa, Noriyuki AU - Ishikawa N FAU - Nakayama, Naomi AU - Nakayama N FAU - Miyazaki, Kohji AU - Miyazaki K LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (ESR1 protein, human) RN - 0 (Estrogen Receptor alpha) RN - 0 (Receptors, Progesterone) SB - IM MH - Endometrial Neoplasms/*genetics/pathology MH - Estrogen Receptor alpha/*genetics/metabolism MH - Female MH - *Gene Amplification MH - Humans MH - In Situ Hybridization, Fluorescence MH - Lymphatic Metastasis MH - Middle Aged MH - Neoplasm Metastasis MH - Receptors, Progesterone/metabolism MH - Retrospective Studies OTO - NOTNLM OT - ER/PR OT - ESR1 OT - endometrial carcinoma OT - gene amplification EDAT- 2013/09/12 06:00 MHDA- 2013/12/16 06:00 CRDT- 2013/09/12 06:00 PHST- 2013/09/12 06:00 [entrez] PHST- 2013/09/12 06:00 [pubmed] PHST- 2013/12/16 06:00 [medline] AID - 33/9/3775 [pii] PST - ppublish SO - Anticancer Res. 2013 Sep;33(9):3775-81.