PMID- 24026226 OWN - NLM STAT- MEDLINE DCOM- 20140407 LR - 20220331 IS - 1791-2431 (Electronic) IS - 1021-335X (Linking) VI - 30 IP - 5 DP - 2013 Nov TI - Cisplatin regulates SH-SY5Y cell growth through downregulation of BDNF via miR-16. PG - 2343-9 LID - 10.3892/or.2013.2731 [doi] AB - Brain-derived neurotropic factor (BDNF) is a member of the neurotropin family. High levels of BDNF are associated with more aggressive malignant behavior in human cancer. In the present study, we observed the effect of cisplatin on BDNF expression in SH-SY5Y cells and investigated the mechanism of cisplatin in inducing the apoptosis of SH-SY5Y cells. Our results revealed that the expression of BDNF was obviously decreased in cisplatin-treated SH-SY5Y cells. In addition, the 3'-untranslated region of BDNF was found to be targeted by miR-16 using microRNA analysis software. After miR-16 was synthesized chemically, SH-SY5Y cells were transfected with miR-16 to investigate the regulatory role of miR-16 in regards to BDNF. The results showed that the expression of BDNF was markedly decreased in the miR‑16-transfected cells when compared with that in the control cultures as determined by western blotting. Moreover, miR-16 expression was obviously upregulated in the cisplatin-treated cells when compared with the untreated controls. Furthermore, SH-SY5Y cells were xenografted subcutaneously in nude mice to study the effect of cisplatin on the growth of SH-SY5Y cells in vivo. The results further showed that cisplatin inhibited the proliferation of SH-SY5Y cells in the cisplatin-treated mice when compared with the saline-treated control. The expression of miR-16 was increased, while the expression of BDNF was decreased in the cisplatin-treated mice. Our results demonstrated that cisplatin downregulated the expression of BDNF through miR-16 to inhibit SH-SY5Y cell proliferation in vitro and in vivo. These findings provide the basis for new targets for drug design or cancer therapy. FAU - Sun, Yun-Xiao AU - Sun YX AD - Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, P.R. China. FAU - Yang, Jian AU - Yang J FAU - Wang, Ping-Yu AU - Wang PY FAU - Li, You-Jie AU - Li YJ FAU - Xie, Shu-Yang AU - Xie SY FAU - Sun, Ruo-Peng AU - Sun RP LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130910 PL - Greece TA - Oncol Rep JT - Oncology reports JID - 9422756 RN - 0 (3' Untranslated Regions) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (MIRN16 microRNA, human) RN - 0 (MicroRNAs) RN - 7171WSG8A2 (BDNF protein, human) RN - Q20Q21Q62J (Cisplatin) SB - IM MH - 3' Untranslated Regions MH - Animals MH - Apoptosis/drug effects MH - Brain-Derived Neurotrophic Factor/biosynthesis/*genetics MH - Cell Cycle/drug effects MH - Cell Line, Tumor MH - Cell Proliferation/drug effects MH - Cisplatin/*administration & dosage MH - Down-Regulation/drug effects MH - Gene Expression Regulation, Neoplastic/drug effects MH - Humans MH - Mice MH - MicroRNAs/*genetics MH - Neuroblastoma/*drug therapy/genetics/pathology MH - Xenograft Model Antitumor Assays EDAT- 2013/09/13 06:00 MHDA- 2014/04/08 06:00 CRDT- 2013/09/13 06:00 PHST- 2013/07/12 00:00 [received] PHST- 2013/08/23 00:00 [accepted] PHST- 2013/09/13 06:00 [entrez] PHST- 2013/09/13 06:00 [pubmed] PHST- 2014/04/08 06:00 [medline] AID - 10.3892/or.2013.2731 [doi] PST - ppublish SO - Oncol Rep. 2013 Nov;30(5):2343-9. doi: 10.3892/or.2013.2731. Epub 2013 Sep 10.