PMID- 24026517 OWN - NLM STAT- MEDLINE DCOM- 20140603 LR - 20171116 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) VI - 405 IP - 27 DP - 2013 Nov TI - Mass spectrometric monitoring of Sr-enriched bone cements--from in vitro to in vivo. PG - 8769-80 LID - 10.1007/s00216-013-7329-8 [doi] AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a well-established technique in materials science, but is now increasingly applied also in the life sciences. Here, we demonstrate the potential of this analytical technique for use in the development of new bone implant materials. We tracked strontium-enriched calcium phosphate cements, which were developed for the treatment of osteoporotic bone, from in vitro to in vivo. Essentially, the spatial distribution of strontium in two different types of strontium-modified calcium phosphate cements is analysed by SIMS depth profiling. To gain information about the strontium release kinetics, the cements were immersed for 3, 7, 14 and 21 days in alpha-MEM and tris(hydroxymethyl)-aminomethane solution and analysed afterwards by ToF-SIMS depth profiling. For cements stored in alpha-MEM solution an inhibited strontium release was observed. By using principal component analysis to evaluate TOF-SIMS surface spectra, we are able to prove the adsorption of proteins on the cement surface, which inhibit the release kinetics. Cell experiments with human osteoblast-like cells cultured on the strontium-modified cements and subsequent mass spectrometric analysis of the mineralised extracellular matrix (mECM) prove clearly that strontium is incorporated into the mECM of the osteoblast-like cells. Finally, in an animal experiment, the strontium-doped cements are implanted into the femur of osteoporotic rats. After 6 weeks, only a slight release of strontium was found in the vicinity of the implant material. By using ToF-SIMS, it is proven that strontium is localised in regions of newly formed bone but also within the pre-existing tissue. FAU - Rohnke, Marcus AU - Rohnke M AD - Institute for Physical Chemistry, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 58, 35392, Giessen, Germany, Marcus.Rohnke@phys.chemie.uni-giessen.de. FAU - Henss, Anja AU - Henss A FAU - Kokesch-Himmelreich, Julia AU - Kokesch-Himmelreich J FAU - Schumacher, Matthias AU - Schumacher M FAU - Ray, Seemun AU - Ray S FAU - Alt, Volker AU - Alt V FAU - Gelinsky, Michael AU - Gelinsky M FAU - Janek, Juergen AU - Janek J LA - eng GR - EB-002027/EB/NIBIB NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20130913 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 RN - 0 (Bone Cements) RN - 0 (Calcium Phosphates) RN - 0 (Organic Chemicals) RN - 0 (Phosphates) RN - 0 (alpha minimal essential medium) RN - 97Z1WI3NDX (calcium phosphate) RN - B7CF3BD0TT (strontium phosphate) RN - YZS2RPE8LE (Strontium) SB - IM MH - Animals MH - Bone Cements/chemistry/*pharmacology MH - Calcium Phosphates/analysis/chemistry/metabolism MH - Cells, Cultured MH - Diffusion MH - Female MH - Femur/chemistry/*drug effects/metabolism/pathology MH - Kinetics MH - Organic Chemicals/chemistry MH - Osteoblasts/cytology/metabolism MH - Osteoporosis/metabolism/pathology/*therapy MH - Ovariectomy MH - Phosphates/*analysis/chemistry/metabolism MH - Principal Component Analysis MH - Prostheses and Implants MH - Rats, Sprague-Dawley MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MH - Strontium/*analysis/chemistry/metabolism EDAT- 2013/09/13 06:00 MHDA- 2014/06/04 06:00 CRDT- 2013/09/13 06:00 PHST- 2013/06/13 00:00 [received] PHST- 2013/08/28 00:00 [accepted] PHST- 2013/08/23 00:00 [revised] PHST- 2013/09/13 06:00 [entrez] PHST- 2013/09/13 06:00 [pubmed] PHST- 2014/06/04 06:00 [medline] AID - 10.1007/s00216-013-7329-8 [doi] PST - ppublish SO - Anal Bioanal Chem. 2013 Nov;405(27):8769-80. doi: 10.1007/s00216-013-7329-8. Epub 2013 Sep 13.