PMID- 24026670
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20211021
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 94
IP  - Pt 12
DP  - 2013 Dec
TI  - The non-primate hepacivirus 5' untranslated region possesses internal ribosomal 
      entry site activity.
PG  - 2657-2663
LID - 10.1099/vir.0.055764-0 [doi]
AB  - The 5' untranslated region (5'UTR) of the recently described non-primate 
      hepacivirus (NPHV) contains a region with sequence homology to the internal 
      ribosomal entry site (IRES) of hepatitis C virus (HCV) and GB virus B (GBV-B). 
      Here, we demonstrated internal translation initiation by the NPHV 5'UTR in a 
      bicistronic vector. An RNA stem-loop upstream of the NPHV IRES was structurally 
      distinct from corresponding regions in HCV and GBV-B, and was not required for 
      IRES function. Insertion of the NPHV stem-loop into the corresponding region of 
      the HCV 5'UTR within the HCV subgenomic replicon significantly impaired RNA 
      replication, indicating that long-range interactions between the 5'UTR and 
      cis-acting downstream elements within the NPHV genome are not interchangeable 
      with those of HCV. Despite similarities in IRES structure and function between 
      hepaciviruses, replication elements in the NPHV 5'UTR appear functionally 
      distinct from those of HCV.
FAU - Stewart, Hazel
AU  - Stewart H
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences and 
      Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 
      9JT, UK.
FAU - Walter, Cheryl
AU  - Walter C
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences and 
      Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 
      9JT, UK.
FAU - Jones, Dale
AU  - Jones D
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences and 
      Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 
      9JT, UK.
FAU - Lyons, Sinead
AU  - Lyons S
AD  - Infection and Immunity Division, The Roslin Institute, University of Edinburgh, 
      Easter Bush, Edinburgh EH25 9RG, UK.
FAU - Simmonds, Peter
AU  - Simmonds P
AD  - Infection and Immunity Division, The Roslin Institute, University of Edinburgh, 
      Easter Bush, Edinburgh EH25 9RG, UK.
FAU - Harris, Mark
AU  - Harris M
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences and 
      Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 
      9JT, UK.
LA  - eng
GR  - 095831/Wellcome Trust/United Kingdom
GR  - 096670/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130911
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*genetics/physiology
MH  - Animals
MH  - Cell Line
MH  - HEK293 Cells
MH  - Hepacivirus/*genetics/metabolism/physiology
MH  - Humans
MH  - Nucleic Acid Conformation
MH  - *Peptide Chain Initiation, Translational
MH  - Primates/genetics/metabolism
MH  - RNA, Viral/genetics/metabolism
MH  - Replicon/genetics/physiology
MH  - Ribosomes/*metabolism
MH  - Virus Replication/genetics
PMC - PMC3836496
EDAT- 2013/09/13 06:00
MHDA- 2014/01/22 06:00
PMCR- 2013/12/01
CRDT- 2013/09/13 06:00
PHST- 2013/09/13 06:00 [entrez]
PHST- 2013/09/13 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - 055764 [pii]
AID - 10.1099/vir.0.055764-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2013 Dec;94(Pt 12):2657-2663. doi: 10.1099/vir.0.055764-0. Epub 2013 
      Sep 11.