PMID- 24027909
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20130913
IS  - 0034-7744 (Print)
IS  - 0034-7744 (Linking)
VI  - 61
IP  - 3
DP  - 2013 Sep
TI  - Plant regeneration from callus cultures of Vitex trifolia (Lamiales: Lamiaceae): 
      a potential medicinal plant.
PG  - 1083-94
AB  - Vitex trifolia is a shrub species with popular use as a medicinal plant, for 
      which leaves, roots and flowers have been reported to heal different distresses. 
      The increasing exploitation of these plants has endangered its conservation, and 
      has importantly justified the use of biotechnological tools for their 
      propagation. Our aim was to present an efficient protocol for plant regeneration 
      through organogenesis; and simultaneously, to analyze the genetic homogeneity of 
      the established clonal lines by Randomly Amplified Polymorphic DNA (RAPD) and 
      Inter Simple Sequence Repeat (ISSR) markers. Plantlet regeneration was achieved 
      in callus cultures derived from stem, leaf and petiole explants of V. trifolia on 
      a differently supplemented Murashige & Skoog medium, and incubated at 25 +/-2 
      degrees C under a light intensity of 61 micromol/m2s from cool white fluorescent 
      lamps and a 16 h photoperiod. The rate of shoot bud regeneration was positively 
      correlated with the concentration of hormones in the nutrient media. Shoot buds 
      regenerated more rapidly from stem and petiole explants as compared to leaf 
      explants on medium containing 11.10 microM BAP in combination with 0.54 
      microMNAA. Addition of 135.74-271.50 microM adenine sulphate (Ads) and 0.72-1.44 
      microM gibberellic acid (GA3) to the culture medium increased the growth of shoot 
      buds. The highest rate of shoot bud regeneration responses was obtained in stem 
      explants using 11.10 microM BAP in combination with 0.54 microM NAA, 271.50 
      microM Ads and 1.44 microM GA3. In vitro rooting of the differentiated shoots was 
      achieved in media containing 1.23 microM indole butyric acid (IBA) with 2% (w/v) 
      sucrose. Regenerated plantlets were successfully established in soil with 86% 
      survival under field condition. Randomly Amplified Polymorphic DNA and Inter 
      Simple Sequence Repeat markers analyses have confirmed the genetic uniformity of 
      the regenerated plantlets derived from the second up to fifth subcultures. This 
      protocol may help in mass propagation and conservation of this important 
      medicinal plant of great therapeutic potential.
FAU - Samantaray, Sanghamitra
AU  - Samantaray S
AD  - Central Rice Research Institute (CRRI), Orissa, India. smitraray@gmail.com
FAU - Bishoyi, Ashok Kumar
AU  - Bishoyi AK
FAU - Maiti, Satyabrata
AU  - Maiti S
LA  - eng
PT  - Journal Article
PL  - Costa Rica
TA  - Rev Biol Trop
JT  - Revista de biologia tropical
JID - 0404267
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - Microsatellite Repeats
MH  - Plant Growth Regulators/pharmacology
MH  - Plant Shoots/drug effects/growth & development
MH  - Plants, Medicinal/classification/drug effects/*physiology
MH  - Random Amplified Polymorphic DNA Technique
MH  - Regeneration/drug effects/*physiology
MH  - Vitex/classification/drug effects/*physiology
EDAT- 2013/09/14 06:00
MHDA- 2013/10/22 06:00
CRDT- 2013/09/14 06:00
PHST- 2013/09/14 06:00 [entrez]
PHST- 2013/09/14 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
PST - ppublish
SO  - Rev Biol Trop. 2013 Sep;61(3):1083-94.